Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes

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dc.contributor.authorMerlos Rodrigo, Miguel Ángelcs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorKomínková, Markétacs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorBeklová, Miroslavacs
dc.contributor.authorZehnálek, Josefcs
dc.contributor.authorKizek, Renécs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue4cs
dc.coverage.volume10cs
dc.date.issued2013-03-28cs
dc.description.abstractIn this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 ug mL 1 PC2 and 100 ug mL 1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexesen
dc.description.abstractIn this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 ug mL 1 PC2 and 100 ug mL 1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexesen
dc.formattextcs
dc.format.extent1304-1311cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Environmental Research and Public Health. 2013, vol. 10, issue 4, p. 1304-1311.en
dc.identifier.doi10.3390/ijerph10041304cs
dc.identifier.issn1660-4601cs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other101373cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/70096
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Environmental Research and Public Healthcs
dc.relation.urihttp://www.mdpi.com/1660-4601/10/4/1304cs
dc.rightsCreative Commons Attribution 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1660-4601/cs
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/cs
dc.subjection exchange chromatographyen
dc.subjectmass spectrometryen
dc.subjectMALDI-TOFen
dc.subjectphytochelatinen
dc.subjectcadmiumen
dc.subjectintramolecular complexen
dc.subjection exchange chromatography
dc.subjectmass spectrometry
dc.subjectMALDI-TOF
dc.subjectphytochelatin
dc.subjectcadmium
dc.subjectintramolecular complex
dc.titleIon exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexesen
dc.title.alternativeIon exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-101373en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 14:11:10en
sync.item.modts2025.10.14 10:49:33en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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