Chytré nanonástroje
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- ItemDemand Response Method for the Domestic PV system(Elsevier, 2024-08-30) Kaczmarczyk, Václav; Arm, Jakub; Baštán, Ondřej; Kaczmarczyková, ZuzanaThis paper describes the deployment of the well-known Demand Response principle in a domestic PV inverter and spot price tariff environment to minimize power purchase costs. The optimization problem is defined as a pure linear program which is solved using a suitable solver. The results are verified using a dataset that was obtained by metering at a real storage site that was managed by an expert on each monitored day.
- ItemMetallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma(Springer Nature, 2021-03-09) Merlos Rodrigo, Miguel Ángel; Michálková, Hana; Strmiska, Vladislav; Casar, Berta; Crespo, Piero; De los Rios, Vivian; Casal Álvarez, José Ignacio; Haddad, Yazan Abdulmajeed Eyadh; Guráň, Roman; Eckschlager, Tomáš; Pokorná, Petra; Heger, Zbyněk; Adam, VojtěchMetallothionein-3 has poorly characterized functions in neuroblastoma. Cisplatin-based chemotherapy is a major regimen to treat neuroblastoma, but its clinical efficacy is limited by chemoresistance. We investigated the impact of human metallothionein-3 (hMT3) up-regulation in neuroblastoma cells and the mechanisms underlying the cisplatin-resistance. We confirmed the cisplatin-metallothionein complex formation using mass spectrometry. Overexpression of hMT3 decreased the sensitivity of neuroblastoma UKF-NB-4 cells to cisplatin. We report, for the first time, cisplatin-sensitive human UKF-NB-4 cells remodelled into cisplatin-resistant cells via high and constitutive hMT3 expression in an in vivo model using chick chorioallantoic membrane assay. Comparative proteomic analysis demonstrated that several biological pathways related to apoptosis, transport, proteasome, and cellular stress were involved in cisplatin-resistance in hMT3 overexpressing UKF-NB-4 cells. Overall, our data confirmed that up-regulation of hMT3 positively correlated with increased cisplatin-chemoresistance in neuroblastoma, and a high level of hMT3 could be one of the causes of frequent tumour relapses.
- ItemCharacterization of carbon dots covered with polyvinylpyrrolidone and polyethylene glycol(ESG, 2015-10-01) Kudr, Jiří; Richtera, Lukáš; Nejdl, Lukáš; Blažková, Iva; Milosavljević, Vedran; Moravec, Zdeněk; Wawrzak, Dorota; Kopel, Pavel; Ruttkay-Nedecký, Branislav; Adam, Vojtěch; Kizek, RenéLuminescent carbon dots are new type of nanomaterial with possible applications in labelling and imaging due to their optoelectronic properties and their superior biocompatibility. The aim of this study was to synthetize two types of C-dots with different polymeric surface modification and to characterize them. Thermal method was used to prepare C-dots with surface stabilization with polyvinylpyrrolidone and polyethylene glycol. Several optical and electrochemical methods were used to characterize the particles. It was found that C-dots-PEG (3 ± 2 nm) possess stronger emission than C-dots-PVP (9 ± 3 nm) within the whole range of excitation wavelengths from 230 to 390 nm. Nevertheless in both cases the portion of C-dots created fluorescent micro-sized particles. Electrochemical impedance spectroscopy revealed that the electrode modified with both types of C-dots significantly increased resistance of bare glassy carbon electrode (C-dots-PVP 34-times and C-dots-PEG 141-times). In addition, it was found that pure polymers and C-dots also exhibit complex concentration dependent behaviour in Brdicka solution measured using hanging mercury drop electrode, whereas low amount added resulted in Co(II) peak (-1.3 V) shift and further resulted in three unresolved peak evolution between -0.5 and -0.9 V.
- ItemA novel ruthenium based coordination compound against pathogenic bacteria(MDPI, 2020-04-10) Sur, Vishma Pratap; Mazumdar, Aninda; Kopel, Pavel; Mukherjee, S.; Vítek, Petr; Michálková, Hana; Vaculovičová, Markéta; Moulick, AmitavaThe current epidemic of antibiotic-resistant infections urges to develop alternatives to less-effective antibiotics. To assess anti-bacterial potential, a novel coordinate compound (RU-S4) was synthesized using ruthenium-Schiff base-benzimidazole ligand, where ruthenium chloride was used as the central atom. RU-S4 was characterized by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. Antibacterial effect of RU-S4 was studied against Staphylococcus aureus (NCTC 8511), vancomycin-resistant Staphylococcus aureus (VRSA) (CCM 1767), methicillin-resistant Staphylococcus aureus (MRSA) (ST239: SCCmecIIIA), and hospital isolate Staphylococcus epidermidis. The antibacterial activity of RU-S4 was checked by growth curve analysis and the outcome was supported by optical microscopy imaging and fluorescence LIVE/DEAD cell imaging. In vivo (balb/c mice) infection model prepared with VRSA (CCM 1767) and treated with RU-S4. In our experimental conditions, all infected mice were cured. The interaction of coordination compound with bacterial cells were further confirmed by cryo-scanning electron microscope (Cryo-SEM). RU-S4 was completely non-toxic against mammalian cells and in mice and subsequently treated with synthesized RU-S4.
- ItemEffect of lycorine on the green algae Chlamydomonas reinhardtii under UV-C irradiation(Mendel University in Brno, 2019-01-02) Koláčková, Martina; Dvořák, Marek; Klejdus, Bořivoj; Húska, DaliborLycorin (LYC) is an inhibitor of the growth in higher plants through the inhibition of the last step of ascorbic acid biosynthesis. Ascorbates are mainly involved in regulation of intracellular levels reactive oxygen species. There is still limit information about its green microalgal toxicity and specificity. Therefore, present experiment was focused on the LYC toxicity on the green microalgae, Chlamydomonas reinhardtii, moreover the cells of microalgae were exposed to UV-C irradiation to increase ROS. The attention was primary given to the antioxidant response. The higher concentrations than 25 M LYC with 30 min UV-C (250 nm) exposure absolutely inhibited the growth. 10 M LYC and 25 M LYC treatment together with UV-C irradiation suppress the growth caused oxidation stress and enhances antioxidant response.