A novel ruthenium based coordination compound against pathogenic bacteria
Loading...
Date
2020-04-10
Authors
Sur, Vishma Pratap
Mazumdar, Aninda
Kopel, Pavel
Mukherjee, S.
Vítek, Petr
Michálková, Hana
Vaculovičová, Markéta
Moulick, Amitava
0000-0002-1175-7194Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Altmetrics
Abstract
The current epidemic of antibiotic-resistant infections urges to develop alternatives to less-effective antibiotics. To assess anti-bacterial potential, a novel coordinate compound (RU-S4) was synthesized using ruthenium-Schiff base-benzimidazole ligand, where ruthenium chloride was used as the central atom. RU-S4 was characterized by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. Antibacterial effect of RU-S4 was studied against Staphylococcus aureus (NCTC 8511), vancomycin-resistant Staphylococcus aureus (VRSA) (CCM 1767), methicillin-resistant Staphylococcus aureus (MRSA) (ST239: SCCmecIIIA), and hospital isolate Staphylococcus epidermidis. The antibacterial activity of RU-S4 was checked by growth curve analysis and the outcome was supported by optical microscopy imaging and fluorescence LIVE/DEAD cell imaging. In vivo (balb/c mice) infection model prepared with VRSA (CCM 1767) and treated with RU-S4. In our experimental conditions, all infected mice were cured. The interaction of coordination compound with bacterial cells were further confirmed by cryo-scanning electron microscope (Cryo-SEM). RU-S4 was completely non-toxic against mammalian cells and in mice and subsequently treated with synthesized RU-S4.
Description
Citation
International Journal of Molecular Sciences. 2020, vol. 21, issue 7, p. 1-18.
https://www.mdpi.com/1422-0067/21/7/2656
https://www.mdpi.com/1422-0067/21/7/2656
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en