Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes
Loading...
Date
Authors
Merlos Rodrigo, Miguel Ángel
Cernei, Natalia Vladimirovna
Komínková, Markéta
Zítka, Ondřej
Beklová, Miroslava
Zehnálek, Josef
Kizek, René
Adam, Vojtěch
Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
0000-0002-8259-1566 Altmetrics
Abstract
In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 ug mL 1 PC2 and 100 ug mL 1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes
In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 ug mL 1 PC2 and 100 ug mL 1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes
In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 ug mL 1 PC2 and 100 ug mL 1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes
Description
Citation
International Journal of Environmental Research and Public Health. 2013, vol. 10, issue 4, p. 1304-1311.
http://www.mdpi.com/1660-4601/10/4/1304
http://www.mdpi.com/1660-4601/10/4/1304
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en
Study field
Comittee
Date of acceptance
Defence
Result of defence
Collections
Endorsement
Review
Supplemented By
Referenced By
Creative Commons license
Except where otherwised noted, this item's license is described as Creative Commons Attribution 3.0 Unported