UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

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dc.contributor.authorAssi, Navidcs
dc.contributor.authorNejdl, Lukášcs
dc.contributor.authorZemánková, Kristýnacs
dc.contributor.authorPavelicová, Kristýnacs
dc.contributor.authorBezděková, Jaroslavacs
dc.contributor.authorMacka, Miroslavcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorVaculovičová, Markétacs
dc.coverage.issue1cs
dc.coverage.volume11cs
dc.date.issued2021-07-05cs
dc.description.abstractIn this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (l-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of l-cysteine in the range of 12-150 mu g/mL was linear with a detection limit of 3.6 mu g/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for l-cysteine compared to N-acetyl-l-cysteine and l-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for l-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.en
dc.formattextcs
dc.format.extent1-11cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2021, vol. 11, issue 1, p. 1-11.en
dc.identifier.doi10.1038/s41598-021-93137-4cs
dc.identifier.issn2045-2322cs
dc.identifier.orcid0000-0001-6332-8917cs
dc.identifier.orcid0000-0002-6792-2574cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0002-6771-1304cs
dc.identifier.other172185cs
dc.identifier.researcheridG-6553-2012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridE-5583-2016cs
dc.identifier.scopus7004295072cs
dc.identifier.urihttp://hdl.handle.net/11012/200921
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttps://doi.org/10.1038/s41598-021-93137-4cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectthiolen
dc.subjectmolecularly imprinted polymeren
dc.subjectpolydopamineen
dc.subjecturineen
dc.titleUV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiolsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-172185en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:06en
sync.item.modts2025.01.17 18:48:35en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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