UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols
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Date
2021-07-05
Authors
Assi, Navid
Nejdl, Lukáš
Zemánková, Kristýna
Pavelicová, Kristýna
Bezděková, Jaroslava
Macka, Miroslav
Adam, Vojtěch
Vaculovičová, Markéta
0000-0001-6332-8917Advisor
Referee
Mark
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Springer Nature
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Abstract
In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (l-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of l-cysteine in the range of 12-150 mu g/mL was linear with a detection limit of 3.6 mu g/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for l-cysteine compared to N-acetyl-l-cysteine and l-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for l-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.
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Peer-reviewed
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en