Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization
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Date
2022-02-09
Authors
Dufková, Kristýna
Bezdíček, Matěj
Čuprová, Kristina
Pantůčková, Dagmar
Jakubíčková, Markéta
Brhelová, Eva
Kocmanová, Iva
Hodová, Silvie
Hanslianova, Markéta
Juren, Tomáš
ORCID
0000-0002-0205-6935Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
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Abstract
Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of clinical manifestations. The main aim of the presented study was to determine and optimize a novel sequencing independent approach that enables molecular typing of S. aureus isolates and elucidates the transmission of emergent clones between patients. In total, 987 S. aureus isolates including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were used to evaluate the novel typing approach combining high-resolution melting (HRM) analysis of multilocus sequence typing (MLST) genes (mini-MLST) and spa gene (spa-HRM). The novel approach’s discriminatory ability was evaluated by whole-genome sequencing (WGS). The clonal relatedness of tested isolates was set by the BURP and BURST approach using spa and MLST data, respectively. Mini-MLST classified the S. aureus isolates into 38 clusters, followed by spa-HRM classifying the isolates into 101 clusters. The WGS proved HRM-based methods to effectively differentiate between related S. aureus isolates. Visualizing evolutionary relationships among different spa-types provided by the BURP algorithm showed comparable results to MLST/mini-MLST clonal clusters. We proved that the combination of mini-MLST and spa-HRM is rapid, reproducible, and cost-efficient. In addition to high discriminatory ability, the correlation between spa evolutionary relationships and mini-MLST clustering allows the variability in population structure to be monitored.
Description
Citation
Microbiology spectrum. 2022, vol. 10, issue 1, p. 1-10.
https://journals.asm.org/doi/10.1128/spectrum.01817-21
https://journals.asm.org/doi/10.1128/spectrum.01817-21
Document type
Peer-reviewed
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Published version
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Language of document
en