Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization

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dc.contributor.authorDufková, Kristýnacs
dc.contributor.authorBezdíček, Matějcs
dc.contributor.authorČuprová, Kristinacs
dc.contributor.authorPantůčková, Dagmarcs
dc.contributor.authorJakubíčková, Markétacs
dc.contributor.authorBrhelová, Evacs
dc.contributor.authorKocmanová, Ivacs
dc.contributor.authorHodová, Silviecs
dc.contributor.authorHanslianova, Markétacs
dc.contributor.authorJuren, Tomášcs
dc.contributor.authorLipový, Břetislavcs
dc.contributor.authorMayer, Jiřícs
dc.contributor.authorLengerová, Martinacs
dc.coverage.issue1cs
dc.coverage.volume10cs
dc.date.issued2022-02-09cs
dc.description.abstractStaphylococcus aureus is a major bacterial human pathogen that causes a wide variety of clinical manifestations. The main aim of the presented study was to determine and optimize a novel sequencing independent approach that enables molecular typing of S. aureus isolates and elucidates the transmission of emergent clones between patients. In total, 987 S. aureus isolates including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were used to evaluate the novel typing approach combining high-resolution melting (HRM) analysis of multilocus sequence typing (MLST) genes (mini-MLST) and spa gene (spa-HRM). The novel approach’s discriminatory ability was evaluated by whole-genome sequencing (WGS). The clonal relatedness of tested isolates was set by the BURP and BURST approach using spa and MLST data, respectively. Mini-MLST classified the S. aureus isolates into 38 clusters, followed by spa-HRM classifying the isolates into 101 clusters. The WGS proved HRM-based methods to effectively differentiate between related S. aureus isolates. Visualizing evolutionary relationships among different spa-types provided by the BURP algorithm showed comparable results to MLST/mini-MLST clonal clusters. We proved that the combination of mini-MLST and spa-HRM is rapid, reproducible, and cost-efficient. In addition to high discriminatory ability, the correlation between spa evolutionary relationships and mini-MLST clustering allows the variability in population structure to be monitored.en
dc.formattextcs
dc.format.extent1-10cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicrobiology spectrum. 2022, vol. 10, issue 1, p. 1-10.en
dc.identifier.doi10.1128/spectrum.01817-21cs
dc.identifier.issn2165-0497cs
dc.identifier.orcid0000-0002-0205-6935cs
dc.identifier.other176720cs
dc.identifier.researcheridR-3181-2018cs
dc.identifier.scopus57202469794cs
dc.identifier.urihttp://hdl.handle.net/11012/203954
dc.language.isoencs
dc.publisherAmerican Society for Microbiologycs
dc.relation.ispartofMicrobiology spectrumcs
dc.relation.urihttps://journals.asm.org/doi/10.1128/spectrum.01817-21cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2165-0497/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectMLSTen
dc.subjectMRSAen
dc.subjectMSSAen
dc.subjecthigh-resolution meltingen
dc.subjectmini-MLSTen
dc.subjectspa-typingen
dc.subjectwhole-genome sequencingen
dc.titleSequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterizationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-176720en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:39:53en
sync.item.modts2025.01.17 16:39:35en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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