Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization
dc.contributor.author | Dufková, Kristýna | cs |
dc.contributor.author | Bezdíček, Matěj | cs |
dc.contributor.author | Čuprová, Kristina | cs |
dc.contributor.author | Pantůčková, Dagmar | cs |
dc.contributor.author | Nykrýnová, Markéta | cs |
dc.contributor.author | Brhelová, Eva | cs |
dc.contributor.author | Kocmanová, Iva | cs |
dc.contributor.author | Hodová, Silvie | cs |
dc.contributor.author | Hanslianova, Markéta | cs |
dc.contributor.author | Juren, Tomáš | cs |
dc.contributor.author | Lipový, Břetislav | cs |
dc.contributor.author | Mayer, Jiří | cs |
dc.contributor.author | Lengerová, Martina | cs |
dc.coverage.issue | 1 | cs |
dc.coverage.volume | 10 | cs |
dc.date.accessioned | 2022-03-14T11:53:29Z | |
dc.date.available | 2022-03-14T11:53:29Z | |
dc.date.issued | 2022-02-09 | cs |
dc.description.abstract | Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of clinical manifestations. The main aim of the presented study was to determine and optimize a novel sequencing independent approach that enables molecular typing of S. aureus isolates and elucidates the transmission of emergent clones between patients. In total, 987 S. aureus isolates including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were used to evaluate the novel typing approach combining high-resolution melting (HRM) analysis of multilocus sequence typing (MLST) genes (mini-MLST) and spa gene (spa-HRM). The novel approach’s discriminatory ability was evaluated by whole-genome sequencing (WGS). The clonal relatedness of tested isolates was set by the BURP and BURST approach using spa and MLST data, respectively. Mini-MLST classified the S. aureus isolates into 38 clusters, followed by spa-HRM classifying the isolates into 101 clusters. The WGS proved HRM-based methods to effectively differentiate between related S. aureus isolates. Visualizing evolutionary relationships among different spa-types provided by the BURP algorithm showed comparable results to MLST/mini-MLST clonal clusters. We proved that the combination of mini-MLST and spa-HRM is rapid, reproducible, and cost-efficient. In addition to high discriminatory ability, the correlation between spa evolutionary relationships and mini-MLST clustering allows the variability in population structure to be monitored. | en |
dc.format | text | cs |
dc.format.extent | 1-10 | cs |
dc.format.mimetype | application/pdf | cs |
dc.identifier.citation | Microbiology spectrum. 2022, vol. 10, issue 1, p. 1-10. | en |
dc.identifier.doi | 10.1128/spectrum.01817-21 | cs |
dc.identifier.issn | 2165-0497 | cs |
dc.identifier.other | 176720 | cs |
dc.identifier.uri | http://hdl.handle.net/11012/203954 | |
dc.language.iso | en | cs |
dc.publisher | American Society for Microbiology | cs |
dc.relation.ispartof | Microbiology spectrum | cs |
dc.relation.uri | https://journals.asm.org/doi/10.1128/spectrum.01817-21 | cs |
dc.rights | Creative Commons Attribution 4.0 International | cs |
dc.rights.access | openAccess | cs |
dc.rights.sherpa | http://www.sherpa.ac.uk/romeo/issn/2165-0497/ | cs |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | cs |
dc.subject | MLST | en |
dc.subject | MRSA | en |
dc.subject | MSSA | en |
dc.subject | high-resolution melting | en |
dc.subject | mini-MLST | en |
dc.subject | spa-typing | en |
dc.subject | whole-genome sequencing | en |
dc.title | Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization | en |
dc.type.driver | article | en |
dc.type.status | Peer-reviewed | en |
dc.type.version | publishedVersion | en |
sync.item.dbid | VAV-176720 | en |
sync.item.dbtype | VAV | en |
sync.item.insts | 2022.05.03 12:54:45 | en |
sync.item.modts | 2022.05.03 12:14:51 | en |
thesis.grantor | Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrství | cs |
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