DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

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dc.contributor.authorHankeová, Simonacs
dc.contributor.authorŠalplachta, Jakubcs
dc.contributor.authorVan Hul, Noemics
dc.contributor.authorKavková, Michaelacs
dc.contributor.authorIqbal, Afsancs
dc.contributor.authorZikmund, Tomášcs
dc.contributor.authorKaiser, Jozefcs
dc.contributor.authorAndersson, Emma Rachelcs
dc.coverage.issuee62941cs
dc.coverage.volume175cs
dc.date.accessioned2021-12-06T11:51:00Z
dc.date.available2021-12-06T11:51:00Z
dc.date.issued2021-09-28cs
dc.description.abstractThe liver is the biggest internal organ in humans and mice, and high auto-fluorescence presents a significant challenge for assessing the three-dimensional (3D) architecture of the organ at the whole-organ level. Liver architecture is characterized by multiple branching lumenized structures, which can be filled with resin, including vascular and biliary trees, establishing a highly stereotyped pattern in the otherwise hepatocyte-rich parenchyma. This protocol describes the pipeline for performing double resin casting micro-computed tomography, or "DUCT". DUCT entails injecting the portal vein and common bile duct with two different radiopaque synthetic resins, followed by tissue fixation. Quality control by clearing one lobe, or the entire liver, with an optical clearing agent, allows for pre-screening of suitably injected samples. In the second part of the DUCT pipeline, a lobe or the whole liver can be used for micro-computed tomography (microCT) scanning, (semi-)automated segmentation, and 3D rendering of the portal venous and biliary networks. MicroCT results in 3D coordinate data for the two resins allowing for qualitative as well as quantitative analysis of the two systems and their spatial relationship. DUCT can be applied to postnatal and adult mouse liver and can be further extended to other tubular networks, for example, vascular networks and airways in the lungs.en
dc.formattextcs
dc.format.extent1-19cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of visualized experiments : JoVE. 2021, vol. 175, issue e62941, p. 1-19.en
dc.identifier.doi10.3791/62941cs
dc.identifier.issn1940-087Xcs
dc.identifier.other172883cs
dc.identifier.urihttp://hdl.handle.net/11012/203065
dc.language.isoencs
dc.publisherMYJoVE Corporationcs
dc.relation.ispartofJournal of visualized experiments : JoVEcs
dc.relation.urihttps://www.jove.com/t/62941/duct-double-resin-casting-followed-micro-computed-tomography-for-3dcs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1940-087X/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectmicro CTen
dc.subjectliver analysisen
dc.subjectDUCTen
dc.titleDUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysisen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-172883en
sync.item.dbtypeVAVen
sync.item.insts2022.05.12 12:53:52en
sync.item.modts2022.05.12 12:14:49en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé instrumentace a metody pro charakterizace materiálůcs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Ústav soudního inženýrství. Ústav soudního inženýrstvícs
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Pokročilé instrumentace a metody pro charakterizace materiálů
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