Formation of G-quadruplex and its utilizing for an automated spectrometric detection of cisplatin

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dc.contributor.authorRuttkay-Nedecký, Branislavcs
dc.contributor.authorSkaličková, Sylviecs
dc.contributor.authorKremplová, Monikacs
dc.contributor.authorNejdl, Lukášcs
dc.contributor.authorKudr, Jiřícs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorNovotná, Mariecs
dc.contributor.authorLabuda, Jancs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue5cs
dc.coverage.volume10cs
dc.date.accessioned2021-09-03T10:52:49Z
dc.date.available2021-09-03T10:52:49Z
dc.date.issued2015-05-29cs
dc.description.abstractHere, we studied the formation of G-quadruplex using spectrophotometric methods (peroxidase activity of G-quadruplex) and verifications of obtained results by voltammetry (cytosine-adenine (CA) or guanine (G) peaks height). The results showed that the peroxidase activity of G-quadruplex increases with the increasing concentrations of K+ ions (1-20 mM) and Na+ ions (1-5 mM) with the highest values observed in the presence of K+ ions. The reverse trend was obtained with NH4+ ions, where the highest peroxidase activity was observed at the lowest concentration of NH4+ ions. The G-quadruplex formation was also proven electrochemically. The addition of 5 mM of K+ ions to the oligonucleotide solution with hemin caused a decrease of both determined peaks. The peroxidase activity of G-quadruplex increased with the increasing concentration of Ag+ ions within the range from 40 nM to 5 mu M. Further, the addition of different concentration of cisplatin (0.5-2 mu M) to the G-quadruplex was monitored. With the addition of cisplatin there was a decrease in peroxidase activity and simultaneously decrease in the CA peak height with the greatest effect in twentieth minute after the addition of cisplatin. The G-quadruplex coupling with gold magnetic nanoparticles was performed and the possibility of automation of spectrophotometric assay was provenen
dc.formattextcs
dc.format.extent3973-3987cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationINTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE. 2015, vol. 10, issue 5, p. 3973-3987.en
dc.identifier.issn1452-3981cs
dc.identifier.other123366cs
dc.identifier.urihttp://hdl.handle.net/11012/201459
dc.language.isoencs
dc.publisherESGcs
dc.relation.ispartofINTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCEcs
dc.relation.urihttp://www.electrochemsci.org/papers/vol10/100503973.pdfcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1452-3981/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectG-quadruplexen
dc.subjectSilver Ionsen
dc.subjectCisplatinen
dc.subjectSpectrophotometric Methodsen
dc.subjectSquare Wave Voltammetryen
dc.subjectCyclic Voltammetryen
dc.titleFormation of G-quadruplex and its utilizing for an automated spectrometric detection of cisplatinen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-123366en
sync.item.dbtypeVAVen
sync.item.insts2021.09.03 12:52:49en
sync.item.modts2021.09.03 12:14:22en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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