Formation of G-quadruplex and its utilizing for an automated spectrometric detection of cisplatin

Abstract
Here, we studied the formation of G-quadruplex using spectrophotometric methods (peroxidase activity of G-quadruplex) and verifications of obtained results by voltammetry (cytosine-adenine (CA) or guanine (G) peaks height). The results showed that the peroxidase activity of G-quadruplex increases with the increasing concentrations of K+ ions (1-20 mM) and Na+ ions (1-5 mM) with the highest values observed in the presence of K+ ions. The reverse trend was obtained with NH4+ ions, where the highest peroxidase activity was observed at the lowest concentration of NH4+ ions. The G-quadruplex formation was also proven electrochemically. The addition of 5 mM of K+ ions to the oligonucleotide solution with hemin caused a decrease of both determined peaks. The peroxidase activity of G-quadruplex increased with the increasing concentration of Ag+ ions within the range from 40 nM to 5 mu M. Further, the addition of different concentration of cisplatin (0.5-2 mu M) to the G-quadruplex was monitored. With the addition of cisplatin there was a decrease in peroxidase activity and simultaneously decrease in the CA peak height with the greatest effect in twentieth minute after the addition of cisplatin. The G-quadruplex coupling with gold magnetic nanoparticles was performed and the possibility of automation of spectrophotometric assay was proven
Description
Citation
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE. 2015, vol. 10, issue 5, p. 3973-3987.
http://www.electrochemsci.org/papers/vol10/100503973.pdf
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Peer-reviewed
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Published version
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en
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Defence
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Creative Commons Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
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