Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection
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Date
2019-01-02
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Advisor
Referee
Mark
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Mendel University in Brno
Abstract
Immunoanalytical techniques are key methods of application in clinical diagnostics, genomics,
proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the
ability of labeled antibodies to bind specific antigens. It is possible to use a large variety of nanomaterials
that are designed, synthesized and adapted to allow highly sensitive detection of advanced
immunoassays. Detection can be a highly efficient analytical method of laser ablation followed by
inductively coupled plasma mass spectrometry (LA-ICP-MS), which allows the detection of elemental
tags suitably conjugated to antibodies. The aim of this work was to conjugate model anti-mouse antibody
on a surface of 10nm and 60nm gold nanoparticles and choose the better one for conjugation
experimentally by using dot-blot immunobinding assay followed by LA-ICP-MS. It has been
experimentally proven that 10nm gold nanoparticles are more suitable for conjugation with antibodies
because of lower non-specific sorption on a membrane.
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Peer-reviewed
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en
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(C) Mendel University in Brno