Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection

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dc.contributor.authorVlčnovská, Marcelacs
dc.contributor.authorTvrdoňová, Michaelacs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorVaculovič, Tomášcs
dc.date.accessioned2019-06-20T06:10:44Z
dc.date.available2019-06-20T06:10:44Z
dc.date.issued2019-01-02cs
dc.description.abstractImmunoanalytical techniques are key methods of application in clinical diagnostics, genomics, proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the ability of labeled antibodies to bind specific antigens. It is possible to use a large variety of nanomaterials that are designed, synthesized and adapted to allow highly sensitive detection of advanced immunoassays. Detection can be a highly efficient analytical method of laser ablation followed by inductively coupled plasma mass spectrometry (LA-ICP-MS), which allows the detection of elemental tags suitably conjugated to antibodies. The aim of this work was to conjugate model anti-mouse antibody on a surface of 10nm and 60nm gold nanoparticles and choose the better one for conjugation experimentally by using dot-blot immunobinding assay followed by LA-ICP-MS. It has been experimentally proven that 10nm gold nanoparticles are more suitable for conjugation with antibodies because of lower non-specific sorption on a membrane.en
dc.formattextcs
dc.format.extent548-552cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2018. 2019, p. 548-552.en
dc.identifier.isbn978-80-7509-597-8cs
dc.identifier.other156248cs
dc.identifier.urihttp://hdl.handle.net/11012/178390
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2018cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201801-0116_Utilization-of-antibody-nanoparticle-conjugates-as-a-tool-for-immunochemistry-with-ICP-MS-detection.php?back=/magno/mnt/2018/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectImmunochemistryen
dc.subjectdot-bloten
dc.subjectgold nanoparticlesen
dc.subjectLA-ICP-MSen
dc.titleUtilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detectionen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-156248en
sync.item.dbtypeVAVen
sync.item.insts2020.04.01 11:01:30en
sync.item.modts2020.04.01 05:50:37en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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