Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection
dc.contributor.author | Vlčnovská, Marcela | cs |
dc.contributor.author | Tvrdoňová, Michaela | cs |
dc.contributor.author | Vaculovičová, Markéta | cs |
dc.contributor.author | Vaculovič, Tomáš | cs |
dc.date.accessioned | 2019-06-20T06:10:44Z | |
dc.date.available | 2019-06-20T06:10:44Z | |
dc.date.issued | 2019-01-02 | cs |
dc.description.abstract | Immunoanalytical techniques are key methods of application in clinical diagnostics, genomics, proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the ability of labeled antibodies to bind specific antigens. It is possible to use a large variety of nanomaterials that are designed, synthesized and adapted to allow highly sensitive detection of advanced immunoassays. Detection can be a highly efficient analytical method of laser ablation followed by inductively coupled plasma mass spectrometry (LA-ICP-MS), which allows the detection of elemental tags suitably conjugated to antibodies. The aim of this work was to conjugate model anti-mouse antibody on a surface of 10nm and 60nm gold nanoparticles and choose the better one for conjugation experimentally by using dot-blot immunobinding assay followed by LA-ICP-MS. It has been experimentally proven that 10nm gold nanoparticles are more suitable for conjugation with antibodies because of lower non-specific sorption on a membrane. | en |
dc.format | text | cs |
dc.format.extent | 548-552 | cs |
dc.format.mimetype | application/pdf | cs |
dc.identifier.citation | MendelNet 2018. 2019, p. 548-552. | en |
dc.identifier.isbn | 978-80-7509-597-8 | cs |
dc.identifier.other | 156248 | cs |
dc.identifier.uri | http://hdl.handle.net/11012/178390 | |
dc.language.iso | en | cs |
dc.publisher | Mendel University in Brno | cs |
dc.relation.ispartof | MendelNet 2018 | cs |
dc.relation.uri | https://mendelnet.cz/artkey/mnt-201801-0116_Utilization-of-antibody-nanoparticle-conjugates-as-a-tool-for-immunochemistry-with-ICP-MS-detection.php?back=/magno/mnt/2018/mn1.php?secid=4 | cs |
dc.rights | (C) Mendel University in Brno | cs |
dc.rights.access | openAccess | cs |
dc.subject | Immunochemistry | en |
dc.subject | dot-blot | en |
dc.subject | gold nanoparticles | en |
dc.subject | LA-ICP-MS | en |
dc.title | Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection | en |
dc.type.driver | conferenceObject | en |
dc.type.status | Peer-reviewed | en |
dc.type.version | publishedVersion | en |
sync.item.dbid | VAV-156248 | en |
sync.item.dbtype | VAV | en |
sync.item.insts | 2020.04.01 11:01:30 | en |
sync.item.modts | 2020.04.01 05:50:37 | en |
thesis.grantor | Vysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástroje | cs |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- Utilization of antibodynanoparticle conjugates as a tool for immunochemistry with ICPMS detection.pdf
- Size:
- 685.54 KB
- Format:
- Adobe Portable Document Format
- Description:
- Utilization of antibodynanoparticle conjugates as a tool for immunochemistry with ICPMS detection.pdf