Porous silica-doped calcium phosphate scaffolds prepared via in-situ foaming method
Loading...
Date
2024-11-01
Authors
Šiška Virágová, Eliška
Novotná, Lenka
Chlup, Zdeněk
Šťastný, Přemysl
Šárfy, Pavlína
Cihlář, Jaroslav
Kučírek, Martin
Benák, Leoš
Streit, Libor
Kocanda, Jan
0009-0004-2648-6763Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
ELSEVIER SCI LTD
Altmetrics
Abstract
The effect of silica (SiO2) addition (0 wt%-20 wt%) on the microstructural and mechanical properties, as well as the in vitro response of calcium phosphate scaffolds for potential application in bone tissue engineering (BTE) was investigated in this research. Scaffolds characterized by high porosity (77%-88 %) and interconnected spherical pores with a broad range of pore sizes (5-600 mu m) were fabricated using in-situ foaming method. Incorporated silica affected the phase transformation of hydroxyapatite (HA) to beta-tricalcium phosphate (beta-TCP) and led to the development of new crystalline silica-rich phases like silicocarnotite and wollastonite. The reinforcement of silica became apparent during the tests of mechanical properties. Scaffolds with 5 wt% of SiO2 exhibited compressive strength (1.13 MPa) higher than pure HA scaffolds (0.93 MPa). Bone bonding potential of the materials was tested in simulated body fluid (SBF), demonstrating this potential in silica-doped samples. Additionally, degradation experiments showed gradual material degradation, making it suitable for BTE applications. Furthermore, cell culture studies using human mesenchymal stromal cells (MSC) confirmed the scaffold's non- toxicity and provided insights into how the silica content influences cell viability, morphology, and osteogenic potential. The findings of this study offer valuable insights into the design and development of advanced scaffolds with tailored properties for effective BTE applications.
Description
Citation
CERAMICS INTERNATIONAL. 2024, vol. 50, issue 21, p. 41215-41227.
https://www.sciencedirect.com/science/article/pii/S0272884224033790?via%3Dihub
https://www.sciencedirect.com/science/article/pii/S0272884224033790?via%3Dihub
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en