Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

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dc.contributor.authorKunishima, Naokics
dc.contributor.authorTakeda, Yoshihirocs
dc.contributor.authorHirose, Raitacs
dc.contributor.authorKalasová, Dominikacs
dc.contributor.authorĹ alplachta, Jakubcs
dc.contributor.authorOmote, Kazuhikocs
dc.coverage.issue1cs
dc.coverage.volume16cs
dc.date.issued2020-02-01cs
dc.description.abstractThe visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) with a phase-retrieval technique. Recently, a laboratory-based X-ray microscope adopting the phase contrast CT was developed as a powerful tool for the observation of weakly absorbing biological samples. Here we report the observation of unstained pansy seeds using the laboratory-based X-ray phase-contrast CT.en
dc.formattextcs
dc.format.extent1-10cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLANT METHODS. 2020, vol. 16, issue 1, p. 1-10.en
dc.identifier.doi10.1186/s13007-020-0557-ycs
dc.identifier.issn1746-4811cs
dc.identifier.orcid0000-0003-3559-9562cs
dc.identifier.orcid0000-0002-0149-7843cs
dc.identifier.other161932cs
dc.identifier.researcheridR-1104-2018cs
dc.identifier.urihttp://hdl.handle.net/11012/193426
dc.language.isoencs
dc.publisherBioMed Centralcs
dc.relation.ispartofPLANT METHODScs
dc.relation.urihttp://link.springer.com/article/10.1186/s13007-020-0557-ycs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1746-4811/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subject3D renderingen
dc.subjectGerminationen
dc.subjectPansy seedsen
dc.subjectTomographyen
dc.subjectX-ray microscopeen
dc.titleVisualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomographyen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161932en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 16:10:34en
sync.item.modts2025.01.17 18:35:06en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé instrumentace a metody pro charakterizace materiálůcs
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Pokročilé instrumentace a metody pro charakterizace materiálů