Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography
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Date
2020-02-01
Authors
Kunishima, Naoki
Takeda, Yoshihiro
Hirose, Raita
Kalasová, Dominika
Ĺ alplachta, Jakub
Omote, Kazuhiko
0000-0003-3559-9562Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
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Abstract
The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) with a phase-retrieval technique. Recently, a laboratory-based X-ray microscope adopting the phase contrast CT was developed as a powerful tool for the observation of weakly absorbing biological samples. Here we report the observation of unstained pansy seeds using the laboratory-based X-ray phase-contrast CT.
Description
Citation
PLANT METHODS. 2020, vol. 16, issue 1, p. 1-10.
http://link.springer.com/article/10.1186/s13007-020-0557-y
http://link.springer.com/article/10.1186/s13007-020-0557-y
Document type
Peer-reviewed
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Published version
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Language of document
en