Bacterial Diversity in the Asphalt Concrete Lining of the Upper Water Reservoir of a Pumped-Storage Scheme

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dc.contributor.authorŠpano, Miroslavcs
dc.contributor.authorŘíha, Jaromírcs
dc.contributor.authorŠpanová, Alenacs
dc.contributor.authorŠedo, Ondrejcs
dc.contributor.authorRittich, Bohuslavcs
dc.coverage.issue11cs
dc.coverage.volume12cs
dc.date.issued2020-10-30cs
dc.description.abstractAsphalt concrete linings (ACLs) are frequently used in pumped-storage hydropower schemes with frequently oscillating reservoir water levels. ACLs can be damaged during operation, which shortens their service life. One type of damage is the formation of blisters. In this study, the bacterial diversity in damaged and undamaged portions of the ACL of an upper water reservoir was evaluated. Matrix-Assisted Laser Desorption/Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) was employed for the identification of the bacterial strains. No bacteria were detected growing inside the undamaged dense layer. Low bacterial diversity (2 and 4 morphotypes) was found in the upper part that has no contact with the reservoir water and in the lowest part that is permanently under water, respectively. More complex bacterial diversity (16 morphotypes covered by 2 different genera and 4 species) was found on the mastic coating of the ACL, which was alternately under and above the water. Very complex bacterial compositions (53 morphotypes covered by at least 13 different species from 6 genera) were found in samples taken from open blisters. Bacterial genera that are known to utilize asphalt products as a source of nutrition were found in the open blisters. The results reflect the importance of bacterial diversity in blisters and their possible role in ACL degradation.en
dc.description.abstractV této studii byla hodnocena bakteriální rozmanitost v poškozených a nepoškozených částech asfaltového betonového obložení ( ACL) Upper Water nádrže. ACL se mohou během provozu poškodit, což zkracuje jejich životnost. Jedním typem poškození je tvorba puchýřů. V otevřených puchýřích byly nalezeny bakteriální rody, o nichž je známo, že používají asfaltové výrobky jako zdroj výživy Výsledky odrážejí význam bakteriální rozmanitosti v blistrech a jejich možné role v degradaci ACL.cs
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationWater. 2020, vol. 12, issue 11, p. 1-15.en
dc.identifier.doi10.3390/w12113045cs
dc.identifier.issn2073-4441cs
dc.identifier.orcid0000-0001-6299-4440cs
dc.identifier.orcid0000-0002-1362-5769cs
dc.identifier.other166173cs
dc.identifier.researcheridAAX-5447-2020cs
dc.identifier.researcheridAAY-4760-2020cs
dc.identifier.scopus16319696800cs
dc.identifier.scopus25823496100cs
dc.identifier.urihttp://hdl.handle.net/11012/195721
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofWatercs
dc.relation.urihttps://www.mdpi.com/2073-4441/12/11/3045cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4441/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpumped-storage hydropower planten
dc.subjectasphalt concrete liningen
dc.subjectopen blistersen
dc.subjectbacterial diversityen
dc.subjectMALDI-TOFMSen
dc.subjectpřečerpávací vodní elektrárna
dc.subjectasfaltobetonové ostění
dc.subjectotevřené puchýře
dc.subjectbakteriální rozmanitost
dc.subjectMALDI-TOFMS
dc.titleBacterial Diversity in the Asphalt Concrete Lining of the Upper Water Reservoir of a Pumped-Storage Schemeen
dc.title.alternativeBakteriální rozmanitost v asfaltové betonové vyzdívce režimu přečerpávání v Upper Water nádržics
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-166173en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:46:17en
sync.item.modts2025.01.17 16:42:05en
thesis.grantorVysoké učení technické v Brně. . Fakulta chemická (HS)cs
thesis.grantorVysoké učení technické v Brně. Fakulta stavební. Ústav vodních stavebcs
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