Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional "amino-glue" surfaces

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dc.contributor.authorJanůšová, Martinacs
dc.contributor.authorJanů, Luciecs
dc.contributor.authorNečas, Davidcs
dc.contributor.authorKřížková, Petracs
dc.contributor.authorBartošíková, Janacs
dc.contributor.authorMedalová, Jiřinacs
dc.contributor.authorKolská, Zdeňkacs
dc.contributor.authorHegemann, Dirkcs
dc.contributor.authorZajíčková, Lenkacs
dc.coverage.issue6cs
dc.coverage.volume20cs
dc.date.issued2023-06-01cs
dc.description.abstractPlasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.en
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPlasma Processes and Polymers. 2023, vol. 20, issue 6, p. 1-15.en
dc.identifier.doi10.1002/ppap.202200157cs
dc.identifier.issn1612-8869cs
dc.identifier.orcid0000-0002-6298-2228cs
dc.identifier.orcid0000-0001-7731-8453cs
dc.identifier.orcid0000-0002-6906-8906cs
dc.identifier.other183943cs
dc.identifier.researcheridD-7166-2012cs
dc.identifier.researcheridE-3010-2012cs
dc.identifier.scopus22933742100cs
dc.identifier.urihttp://hdl.handle.net/11012/213691
dc.language.isoencs
dc.publisherWiley-VCHcs
dc.relation.ispartofPlasma Processes and Polymerscs
dc.relation.urihttps://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157cs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1612-8869/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectamine plasma polymeren
dc.subjectcarboxyl plasma polymeren
dc.subjectcell adhesionen
dc.subjectglycocalyxen
dc.subjectnonspecific adhesionen
dc.titleInsight into peculiar adhesion of cells to plasma-chemically prepared multifunctional "amino-glue" surfacesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-183943en
sync.item.dbtypeVAVen
sync.item.insts2024.02.26 08:46:08en
sync.item.modts2024.02.26 08:13:33en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Plazmové technologie pro materiálycs
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