Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional "amino-glue" surfaces
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Date
2023-06-01
Authors
Janůšová, Martina
Janů, Lucie
Nečas, David
Křížková, Petra
Bartošíková, Jana
Medalová, Jiřina
Kolská, Zdeňka
Hegemann, Dirk
Zajíčková, Lenka
0000-0002-6298-2228Advisor
Referee
Mark
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Publisher
Wiley-VCH
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Abstract
Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.
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Citation
Plasma Processes and Polymers. 2023, vol. 20, issue 6, p. 1-15.
https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157
https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157
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Peer-reviewed
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Published version
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en
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/