Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion.

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dc.contributor.authorČolláková, Janacs
dc.contributor.authorKřížová, Anetacs
dc.contributor.authorKollárová, Věracs
dc.contributor.authorDostál, Zbyněkcs
dc.contributor.authorSlabá, Michalacs
dc.contributor.authorVeselý, Pavelcs
dc.contributor.authorChmelík, Radimcs
dc.coverage.issue11cs
dc.coverage.volume20cs
dc.date.accessioned2020-08-04T11:02:42Z
dc.date.available2020-08-04T11:02:42Z
dc.date.issued2015-11-01cs
dc.description.abstractCoherence-controlled holographic microscopy (CCHM) in low-coherence mode possesses pronounced coherence gate effect. This offers an option to investigate the details of cellular events leading to cell death caused by cytopathic turbid emulsions. CCHM capacity was first assessed in model situations that showed clear images obtained with low coherence of illumination but not with high coherence of illumination. Then, the form of death of human cancer cells induced by treatment with biologically active phospholipids (BAPs) preparation was investigated. The observed overall retraction of cell colony was apparently caused by the release of cell-to-substratum contacts. This was followed by the accumulation of granules decorating the nuclear membrane. Then, the occurrence of nuclear membrane indentations signaled the start of damage to the integrity of the cell nucleus. In the final stage, cells shrunk and disintegrated. This indicated that BAPs cause cell death by necrosis and not apoptosis. An intriguing option of checking the fate of cancer cells caused by the anticipated cooperative effect after adding another tested substance sodium dichloroacetate to turbid emulsion is discussed on grounds of pilot experiments. Such observations should reveal the impact and mechanism of action of the interacting drugs on cell behavior and fate that would otherwise remain hidden in turbid milieu.en
dc.description.abstractKoherencí řízený holografický mikroskop v nízkokoherentním modu vykazuje efekt koherenční brány. Tato vlastnost umožňuje zkoumání detailů buněčného chování a průběhu buněčné smrti způsobené cytopatickým působením turbidní emulze.cs
dc.formattextcs
dc.format.extent111213-1-111213-7cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJOURNAL OF BIOMEDICAL OPTICS. 2015, vol. 20, issue 11, p. 111213-1-111213-7.en
dc.identifier.doi10.1117/1.JBO.20.11.111213cs
dc.identifier.issn1083-3668cs
dc.identifier.other115945cs
dc.identifier.urihttp://hdl.handle.net/11012/84154
dc.language.isoencs
dc.publisherJournal of Biomedical Optics SPIEcs
dc.relation.ispartofJOURNAL OF BIOMEDICAL OPTICScs
dc.relation.urihttp://dx.doi.org/10.1117/1.JBO.20.11.111213cs
dc.rightsCreative Commons Attribution 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1083-3668/cs
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/cs
dc.subjectquantitative phase imaging; holographic microscopy; imaging through turbid media; coherence gate effect; live cell imagingen
dc.subjectcancer cell necrotic deathen
dc.titleCoherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion.en
dc.title.alternativeKoherencí řízený holografický mikroskop umožňuje rozpoznání nekrózy jako typu smrti rakovinných buněk po vystavení cytopatické turbidní emulze.cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-115945en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:02:42en
sync.item.modts2020.08.04 12:26:04en
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Fakulta strojního inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Experimentální biofotonikacs
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