Fakulta strojního inženýrství

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Advanced Microscopical Non-Invasive Examination of the Supposed Migrastatics for Impact on In Vitro Cell Migration
(European Conference of Oncology Pharmacy, 2022-07-02) Šuráňová, Markéta; Zábranská, Magdaléna; Kolínková, Veronika; Muchová, Nikola; Jůzová, Veronika; Chmelík, Radim; Veselý, Pavel
Live H1299 lung carcinoma cells in vitro were exposed to selected drugs with a presumed antimigration activity that implies antimetastatic potential and time-lapse examined with Coherence Controlled Holography Microscopy (CCHM) with holographic incoherent light Quantitive Phase Imaging (hiQPI). It is a methodology that online evaluates the dynamics of morphology, migration, and the growth of tumor cells by weighing them. Material and method: Q-Phase (Telight, Brno, Czech Republic) as a commercially available CCHM was employed for the hiQPI of cells. Four putative migrastatics, vincristine (VIN, 100 nM), doxycycline (DOXY, 1 mg/ml), and 4-hydroxyacetophenone (4HAP, 4 M) were tested with H1299, using Ibidi -Slide VI 0.4 for 20 hours recording with Q-Phase. Cells were cultivated at 37°C in a humidified incubator with 3.5% CO2 in standard Eagle MEM medium with 10% fetal bovine serum, 20 M gentamicin, and 2mM L-glutamine. For the time-lapse recording, the medium was enriched with 20 mM HEPES to maintain pH 7.4. Results and discussion: This research showed that on the cancer cell line H1299 the vincristine and doxycycline had the greatest migrastatic effect in the 2D environment under given conditions. These putative migrastatics showed an effect on the dynamics of migration and cell morphology. Conclusion: The hiQPI screening is a reliable and economical approach to in vitro introductory testing of potential migrastatics. hiQPI combines high-precision cell imaging, which is important for cell segmentation and thus tracking cell trajectories, with cell growth measurements, thus providing a comprehensive assessment of a potential risk of some cytopathic issues.
Overview of the Current State of Gallium Arsenide-Based Solar Cells
(MDPI, 2021-06-04) Papež, Nikola; Dallaev, Rashid; Ţălu, Ştefan; Kaštyl, Jaroslav
As widely-available silicon solar cells, the development of GaAs-based solar cells has been ongoing for many years. Although cells on the gallium arsenide basis today achieve the highest efficiency of all, they are not very widespread. They have particular specifications that make them attractive, especially for certain areas. Thanks to their durability under challenging conditions, it is possible to operate them in places where other solar cells have already undergone significant degradation. This review summarizes past, present, and future uses of GaAs photovoltaic cells. It examines advances in their development, performance, and various current implementations and modifications.
SEM imaging and XPS characterization of doped PVDF fibers
(EDP Sciences, 2021-06-10) Smejkalová, Tereza; Ţălu, Ştefan; Dallaev, Rashid; Částková, Klára; Sobola, Dinara; Nazarov, Anton
Polyvinylidene fluoride (PVDF) is one of the most promising electroactive polymers; it exhibits excellent electroactive behaviours, good biocompatibility, excellent chemical resistance, and thermal stability, rendering it an attractive material for biomedical, electronic, environmental and energy harvesting applications. This work aims to further improve its properties by the inclusion of powders of piezoactive materials. Polyvinylidene fluoride was formed by electrospinning into fibres with a thickness of 1.5-0.3 µm and then examined in a scanning electron microscope. The work offers a description of the current procedure in the preparation of samples and their modification for examination in a scanning electron microscope, characterizes the individual components of doped fibres and deals with specific instruments used for various analytical methods. The work contains a theoretical introduction to the analytical methods to which the samples will be further subjected, such as energy dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS). The obtained excellent properties of doped PVDF could be used in the design of sensors
Preparation of PVDF-CNT composite
(EDP Sciences, 2021-06-09) Misiurev, Denis; Ţălu, Ştefan; Dallaev, Rashid; Sobola, Dinara; Goncharova, Mariya
Limitations of ceramic piezomaterials (brittleness, toxicity of lead-containing samples, difficulties of complicated shapes preparations, etc.) call for the research in the field of piezoelectric polymers. One of them is polyvinylidene fluoride (PVDF). It could be prepared in various forms: thin films, bulk samples, fibers. PVDF fibers attract the most attention because of high flexibility, lightweight, mechanical stability, chemical inertness. Properties of PVDF fibers can be tuned using dopant material: ceramic particles, metal nanoparticles, graphite materials as graphene oxide or carbon nanotubes (CNT).
Step-edge assisted large scale FeSe monolayer growth on epitaxial Bi(2)Se(3)thin films
(IOP Publishing, 2020-07-01) Fikáček, Jan; Procházka, Pavel; Stetsovych, Vitalii; Průša, Stanislav; Vondráček, Martin; Kormoš, Lukáš; Skála, Tomáš; Vlaic, Petru; Caha, Ondřej; Carva, Karel; Čechal, Jan; Springholz, Gunther; Honolka, Jan
Enhanced superconductivity of FeSe in the 2D limit on oxide surfaces as well as the prediction oftopological superconductivityat the interface to topological insulators makes the fabrication of Fe-chalcogenide monolayers a topic of current interest. So far superconductive properties of the latter are mostly studied by scanning tunneling spectroscopy, which can detect gaps in the local density of states as an indicator for Cooper pairing. Direct macroscopic transport properties, which can prove or falsify a true superconducting phase, are yet widely unexplored due to the difficulty to grow monolayer films with homogeneous material properties on a larger scale. Here we report on a promising route to fabricate micron-scale continuous carpets of monolayer thick FeSe on Bi(2)Se(3)topological insulators. In contrast to previous procedures based on ultraflat bulk Bi(2)Se(3)surfaces, we use molecular beam epitaxy grown Bi(2)Se(3)films with high step-edge densities (terrace widths 10-100 nm). We observe that step edges promote the almost strainless growth of coalescing FeSe domains without compromising the underlying Bi(2)Se(3)crystal structure.

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