LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis

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dc.contributor.authorVlčnovská, Marcelacs
dc.contributor.authorKuchynka, Michaelacs
dc.contributor.authorŠtossová, Anetacs
dc.contributor.authorPolanská, Hanacs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorVaculovič, Tomášcs
dc.contributor.authorMasařík, Michalcs
dc.date.issued2019-12-31cs
dc.description.abstractLaser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.en
dc.description.abstractLaser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.en
dc.formattextcs
dc.format.extent682-686cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 682-686.en
dc.identifier.isbn978-80-7509-688-3cs
dc.identifier.orcid0000-0002-1686-4423cs
dc.identifier.orcid0000-0002-6771-1304cs
dc.identifier.orcid0000-0003-1172-7195cs
dc.identifier.other161435cs
dc.identifier.researcheridABE-5364-2021cs
dc.identifier.researcheridE-5583-2016cs
dc.identifier.researcheridD-9920-2012cs
dc.identifier.scopus53464444600cs
dc.identifier.urihttp://hdl.handle.net/11012/184138
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2019 Proceedings of 26th International PhD Students Conferencecs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectgold nanoparticlesen
dc.subjectLA-ICP-MSen
dc.subjectimmunochemistryen
dc.subjectdot-bloten
dc.subjectprotein detectionen
dc.subjectp53en
dc.subjectgold nanoparticles
dc.subjectLA-ICP-MS
dc.subjectimmunochemistry
dc.subjectdot-blot
dc.subjectprotein detection
dc.subjectp53
dc.titleLA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysisen
dc.title.alternativeLA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysisen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161435en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:16:32en
sync.item.modts2025.10.14 10:04:49en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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