LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis

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dc.contributor.authorVlčnovská, Marcelacs
dc.contributor.authorKuchynka, Michaelacs
dc.contributor.authorŠtossová, Anetacs
dc.contributor.authorPolanská, Hanacs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorVaculovič, Tomášcs
dc.contributor.authorMasařík, Michalcs
dc.date.issued2019-12-31cs
dc.description.abstractLaser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.en
dc.formattextcs
dc.format.extent682-686cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 682-686.en
dc.identifier.isbn978-80-7509-688-3cs
dc.identifier.orcid0000-0002-1686-4423cs
dc.identifier.orcid0000-0002-6771-1304cs
dc.identifier.orcid0000-0003-1172-7195cs
dc.identifier.other161435cs
dc.identifier.researcheridABE-5364-2021cs
dc.identifier.researcheridE-5583-2016cs
dc.identifier.researcheridD-9920-2012cs
dc.identifier.scopus53464444600cs
dc.identifier.urihttp://hdl.handle.net/11012/184138
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2019 Proceedings of 26th International PhD Students Conferencecs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectgold nanoparticlesen
dc.subjectLA-ICP-MSen
dc.subjectimmunochemistryen
dc.subjectdot-bloten
dc.subjectprotein detectionen
dc.subjectp53en
dc.titleLA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysisen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161435en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:55en
sync.item.modts2025.01.17 18:40:16en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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