Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification

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dc.contributor.authorPranada, Arthur B.cs
dc.contributor.authorČičatka, Michalcs
dc.contributor.authorHeß, Claracs
dc.contributor.authorKarásek, Jancs
dc.coverage.issue09-2024cs
dc.date.accessioned2024-10-14T09:03:31Z
dc.date.available2024-10-14T09:03:31Z
dc.date.issued2024-09-19cs
dc.description.abstractThe MBT Pathfinder® is an automated colony-picking robot designed for efficient sample preparation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. This article presents results from three key experiments evaluating the instrument's performance in conjunction with MALDI Biotyper® instrument. The method comparison experiment assessed its clinical performance, demonstrating comparable results with Gram-positive, Gram-negative, and anaerobic bacteria (scores larger than 2.00) and superior performance over simple direct yeast transfer (score: 1.80) when compared to samples prepared manually. The repeatability experiment confirmed consistent performance over multiple days and labs (average log score: 2.12, std. deviation: 0.59). The challenge panel experiment showcased its consistent and accurate performance across various samples and settings, yielding average scores between 1.76 and 2.19. These findings underline the MBT Pathfinder® as a reliable and efficient tool for MALDI-TOF mass spectrometry sample preparation in clinical and research applications.en
dc.formattextcs
dc.format.extent1-12cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJOURNAL OF CLINICAL MICROBIOLOGY. 2024, issue 09-2024, p. 1-12.en
dc.identifier.doi10.1128/jcm.00434-24cs
dc.identifier.issn0095-1137cs
dc.identifier.orcid0000-0002-8119-6679cs
dc.identifier.other189446cs
dc.identifier.researcheridJDC-4257-2023cs
dc.identifier.scopus58781281100cs
dc.identifier.urihttps://hdl.handle.net/11012/249488
dc.language.isoencs
dc.publisherAmerican Society for Microbiologycs
dc.relation.ispartofJOURNAL OF CLINICAL MICROBIOLOGYcs
dc.relation.urihttps://journals.asm.org/doi/10.1128/jcm.00434-24cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0095-1137/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectagar platesen
dc.subjectsynthetic image generationen
dc.subjectdeep learningen
dc.subjectsemantic segmentationen
dc.titleDiagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identificationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
eprints.grantNumberinfo:eu-repo/grantAgreement/MV0/VK/VK01010153cs
sync.item.dbidVAV-189446en
sync.item.dbtypeVAVen
sync.item.insts2024.10.14 11:03:31en
sync.item.modts2024.09.25 14:32:05en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav telekomunikacícs
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