Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification
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Date
2024-09-19
Authors
Pranada, Arthur B.
Čičatka, Michal
Heß, Clara
Karásek, Jan
ORCID
0000-0002-8119-6679Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
Altmetrics
Abstract
The MBT Pathfinder® is an automated colony-picking robot designed for efficient sample preparation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. This article presents results from three key experiments evaluating the instrument's performance in conjunction with MALDI Biotyper® instrument. The method comparison experiment assessed its clinical performance, demonstrating comparable results with Gram-positive, Gram-negative, and anaerobic bacteria (scores larger than 2.00) and superior performance over simple direct yeast transfer (score: 1.80) when compared to samples prepared manually. The repeatability experiment confirmed consistent performance over multiple days and labs (average log score: 2.12, std. deviation: 0.59). The challenge panel experiment showcased its consistent and accurate performance across various samples and settings, yielding average scores between 1.76 and 2.19. These findings underline the MBT Pathfinder® as a reliable and efficient tool for MALDI-TOF mass spectrometry sample preparation in clinical and research applications.
Description
Citation
JOURNAL OF CLINICAL MICROBIOLOGY. 2024, issue 09-2024, p. 1-12.
https://journals.asm.org/doi/10.1128/jcm.00434-24
https://journals.asm.org/doi/10.1128/jcm.00434-24
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en