Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles

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dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorHorký, Pavelcs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorAdam, Vojtěchcs
dc.date.issued2020-06-10cs
dc.description.abstractIn this paper, we report the optimal conditions for the isolation and preconcentration of branched chain amino acids (BCAAs) - leucine, valine and arginine from different types of matrix, such as tissue, plasma, blood, bacteria or cells. Herein, we focused on the synthesis of paramagnetic particles able to isolate and immobilize amino acids and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified nanomaghemite (-Fe2O3) by multi walled carbon nanotubes (MWCNT) and hyaluronic acid as the functional carriers providing excellent affinity properties. Our paramagnetic particles have potential for better isolation of BCAAs mainly from plasma or bacteria and in the future they can also be applied as a platform of delivery system.en
dc.formattextcs
dc.format.extent374-378cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNANOCON 2019 CONFERENCE PROCEEDINGS. 2020, p. 374-378.en
dc.identifier.doi10.37904/nanocon.2019.8497cs
dc.identifier.isbn978-80-87294-95-6cs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.orcid0000-0002-7318-6470cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other164259cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.researcheridE-5702-2012cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus6603604023cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/196672
dc.language.isoencs
dc.publisherTangercs
dc.relation.ispartofNANOCON 2019 CONFERENCE PROCEEDINGScs
dc.relation.urihttps://doi.org/10.37904/nanocon.2019.8497cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectIon Exchange Chromatographyen
dc.subjectMaghemiteen
dc.subjectMWCNTen
dc.subjectBCAAsen
dc.subjectvalineen
dc.subjectleucineen
dc.subjectarginineen
dc.titleIsolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particlesen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-164259en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:03en
sync.item.modts2025.01.17 18:44:25en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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