Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles

Abstract

In this paper, we report the optimal conditions for the isolation and preconcentration of branched chain amino acids (BCAAs) - leucine, valine and arginine from different types of matrix, such as tissue, plasma, blood, bacteria or cells. Herein, we focused on the synthesis of paramagnetic particles able to isolate and immobilize amino acids and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified nanomaghemite (-Fe2O3) by multi walled carbon nanotubes (MWCNT) and hyaluronic acid as the functional carriers providing excellent affinity properties. Our paramagnetic particles have potential for better isolation of BCAAs mainly from plasma or bacteria and in the future they can also be applied as a platform of delivery system.In this paper, we report the optimal conditions for the isolation and preconcentration of branched chain amino acids (BCAAs) - leucine, valine and arginine from different types of matrix, such as tissue, plasma, blood, bacteria or cells. Herein, we focused on the synthesis of paramagnetic particles able to isolate and immobilize amino acids and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified nanomaghemite (-Fe2O3) by multi walled carbon nanotubes (MWCNT) and hyaluronic acid as the functional carriers providing excellent affinity properties. Our paramagnetic particles have potential for better isolation of BCAAs mainly from plasma or bacteria and in the future they can also be applied as a platform of delivery system.