Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate

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dc.contributor.authorSlavík, Jancs
dc.contributor.authorČmiel, Vratislavcs
dc.contributor.authorHubálek, Jaromírcs
dc.contributor.authorYang, Yics
dc.contributor.authorRen, Tian-Lingcs
dc.coverage.issue1cs
dc.coverage.volume11cs
dc.date.accessioned2021-01-13T11:54:19Z
dc.date.available2021-01-13T11:54:19Z
dc.date.issued2020-12-30cs
dc.description.abstractAlignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene cues on quartz substrate was evaluated in this work. The neurons’ alignment on quartz grooves is considered to be topographical alignment, while the neurons’ alignment on parylene cues on quartz substrate is considered to be chemical alignment. Both quartz grooves’ and parylene cues’ widths were fabricated in a range from 2 µm to 8 µm; quartz grooves’ heights were in a range from 0.25 µm to 4 µm, while parylene cues’ heights were only 0.25 µm. Neurons were dissociated hippocampal neurons from rat E18. Neurons were cultivated on test substrates for 7 days before alignment evaluation. As expected, neurons aligned according to the direction of grooves and cues; however, transversal growth direction was also observed with much smaller tendency. Chemical alignment was found to be more effective than topographical alignment. If parylene cues are thin and distanced enough, then neurons have a tendency to follow the direction of individual parylene cues; however, neurons on quartz grooves have a tendency just to follow a preferable direction than individual quartz grooves.en
dc.formattextcs
dc.format.extent1-12cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationApplied Sciences - Basel. 2020, vol. 11, issue 1, p. 1-12.en
dc.identifier.doi10.3390/app11010275cs
dc.identifier.issn2076-3417cs
dc.identifier.other167392cs
dc.identifier.urihttp://hdl.handle.net/11012/195846
dc.language.isoencs
dc.publisherMDPI AGcs
dc.relation.ispartofApplied Sciences - Baselcs
dc.relation.urihttps://www.mdpi.com/2076-3417/11/1/275cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-3417/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectneuronsen
dc.subjectquartz groovesen
dc.subjectparylene cuesen
dc.subjectneurons alighmenten
dc.subjectneurons analysisen
dc.titleHippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrateen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-167392en
sync.item.dbtypeVAVen
sync.item.insts2021.02.23 07:56:25en
sync.item.modts2021.02.23 00:15:16en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
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