Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues

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dc.contributor.authorŠindelářová, Annacs
dc.contributor.authorPořízka, Pavelcs
dc.contributor.authorModlitbová, Pavlínacs
dc.contributor.authorVrlíková, Luciecs
dc.contributor.authorKiss, Kateřinacs
dc.contributor.authorKaška, Milancs
dc.contributor.authorProchazka, Davidcs
dc.contributor.authorVrábel, Jakubcs
dc.contributor.authorBuchtová, Marcelacs
dc.contributor.authorKaiser, Jozefcs
dc.coverage.issue3cs
dc.coverage.volume21cs
dc.date.accessioned2021-04-10T22:55:14Z
dc.date.available2021-04-10T22:55:14Z
dc.date.issued2021-01-29cs
dc.description.abstractThe improving performance of the laser-induced breakdown spectroscopy (LIBS) triggered its utilization in the challenging topic of soft tissue analysis. Alterations of elemental content within soft tissues are commonly assessed and provide further insights in biological research. However, the laser ablation of soft tissues is a complex issue and demands a priori optimization, which is not straightforward in respect to a typical LIBS experiment. Here, we focus on implementing an internal standard into the LIBS elemental analysis of soft tissue samples. We achieve this by extending routine methodology for optimization of soft tissues analysis with a standard spiking method. This step enables a robust optimization procedure of LIBS experimental settings. Considering the implementation of LIBS analysis to the histological routine, we avoid further alterations of the tissue structure. Therefore, we propose a unique methodology of sample preparation, analysis, and subsequent data treatment, which enables the comparison of signal response from heterogenous matrix for different LIBS parameters. Additionally, a brief step-by-step process of optimization to achieve the highest signal-to-noise ratio (SNR) is described. The quality of laser–tissue interaction is investigated on the basis of the zinc signal response, while selected experimental parameters (e.g., defocus, gate delay, laser energy, and ambient atmosphere) are systematically modified.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2021, vol. 21, issue 3, p. 1-13.en
dc.identifier.doi10.3390/s21030900cs
dc.identifier.issn1424-8220cs
dc.identifier.other169460cs
dc.identifier.urihttp://hdl.handle.net/11012/196339
dc.language.isoencs
dc.publisherSensorscs
dc.relation.ispartofSENSORScs
dc.relation.urihttps://www.mdpi.com/1424-8220/21/3/900/htmcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectlaser-induced breakdown spectroscopyen
dc.subjectelemental mappingen
dc.subjectsoft tissue ablationen
dc.subjectzincen
dc.subjectmurine kidneysen
dc.titleMethodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissuesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-169460en
sync.item.dbtypeVAVen
sync.item.insts2021.04.11 00:55:14en
sync.item.modts2021.04.11 00:15:17en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Charakterizace materiálů a pokročilé povlaky 1-06cs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Ústav soudního inženýrství. Ústav soudního inženýrstvícs
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Charakterizace materiálů a pokročilé povlaky 1-06
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