Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping

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dc.contributor.authorMichálková, Hanacs
dc.contributor.authorStrmiska, Vladislavcs
dc.contributor.authorŠkubalová, Zuzanacs
dc.contributor.authorDostálová, Simonacs
dc.contributor.authorMichálek, Petrcs
dc.contributor.authorKřížková, Soňacs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorKalina, Lukášcs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorMoulick, Amitavacs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorHeger, Zbyněkcs
dc.coverage.issue43cs
dc.coverage.volume16cs
dc.date.issued2018-04-19cs
dc.description.abstractBackground: Suitable fluorophores are the core of fluorescence imaging. Among the most exciting, yet controversial, labels are quantum dots (QDs) with their unique optical and chemical properties, but also considerable toxicity. This hinders QDs applicability in living systems. Surface chemistry has a profound impact on biological behavior of QDs. This study describes a two-step synthesis of QDs formed by CdTe core doped with Schiff base ligand for lanthanides [Ln (Yb3+, Tb3+ and Gd3+)] as novel cytocompatible fluorophores. Results: Microwave-assisted synthesis resulted in water-soluble nanocrystals with high colloidal and fluorescence stability with quantum yields of 40.9-58.0%. Despite induction of endocytosis and cytoplasm accumulation of Yb- and TbQDs, surface doping resulted in significant enhancement in cytocompatibility when compared to the un-doped CdTe QDs. Furthermore, only negligible antimigratory properties without triggering formation of reactive oxygen species were found, particularly for TbQDs. Ln-doped QDs did not cause observable hemolysis, adsorbed only a low degree of plasma proteins onto their surface and did not possess significant genotoxicity. To validate the applicability of Ln-doped QDs for in vitro visualization of receptor status of living cells, we performed a site-directed conjugation of antibodies towards immuno-labeling of clinically relevant target-human norepinephrine transporter (hNET), over-expressed in neuroendocrine tumors like neuroblastoma. Immuno-performance of modified TbQDs was successfully tested in distinct types of cells varying in hNET expression and also in neuroblastoma cells with hNET expression up-regulated by vorinostat. Conclusion: For the first time we show that Ln-doping of CdTe QDs can significantly alleviate their cytotoxic effects. The obtained results imply great potential of Ln-doped QDs as cytocompatible and stable fluorophores for various bio-labeling applications.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of Nanobiotechnology. 2018, vol. 16, issue 43, p. 1-14.en
dc.identifier.doi10.1186/s12951-018-0369-7cs
dc.identifier.issn1477-3155cs
dc.identifier.orcid0000-0002-7036-1640cs
dc.identifier.orcid0000-0002-0121-0128cs
dc.identifier.orcid0000-0002-0479-8369cs
dc.identifier.orcid0000-0002-7318-6470cs
dc.identifier.orcid0000-0001-8127-8175cs
dc.identifier.orcid0000-0002-8288-3999cs
dc.identifier.orcid0000-0001-5769-6748cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0002-3915-7270cs
dc.identifier.other147671cs
dc.identifier.researcheridH-2870-2018cs
dc.identifier.researcheridB-9180-2014cs
dc.identifier.researcheridE-9617-2012cs
dc.identifier.researcheridE-5702-2012cs
dc.identifier.researcheridN-9991-2014cs
dc.identifier.researcheridI-9677-2016cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridD-1973-2013cs
dc.identifier.scopus55255338000cs
dc.identifier.scopus12040049600cs
dc.identifier.scopus55783172600cs
dc.identifier.urihttp://hdl.handle.net/11012/80177
dc.language.isoencs
dc.publisherBioMed Centralcs
dc.relation.ispartofJournal of Nanobiotechnologycs
dc.relation.urihttp://link.springer.com/article/10.1186/s12951-018-0369-7cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1477-3155/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectCellular labelingen
dc.subjectCytotoxicityen
dc.subjectInorganic fluorophoreen
dc.subjectNanocrystalen
dc.subjectSurface dopanten
dc.titleImproving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface dopingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-147671en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:02en
sync.item.modts2025.01.17 15:35:14en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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