An integrated microfluidic platform for nucleic acid testing

Simple item page
dc.contributor.authorSun, Antaocs
dc.contributor.authorVopařilová, Petracs
dc.contributor.authorLiu, Xiaochengcs
dc.contributor.authorKou, Bingqiancs
dc.contributor.authorŘezníček, Tomášcs
dc.contributor.authorLednický, Tomášcs
dc.contributor.authorNi, Shengcs
dc.contributor.authorKudr, Jiřícs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorFohlerová, Zdenkacs
dc.contributor.authorPajer, Petrcs
dc.contributor.authorZhang, Haoqingcs
dc.contributor.authorNeužil, Pavelcs
dc.coverage.issue1cs
dc.coverage.volume10cs
dc.date.issued2024-05-23cs
dc.description.abstractThis study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics. The system integrates the extraction and purification of nucleic acids, followed by amplification via either reverse transcription-quantitative polymerase chain reaction (RT–qPCR) or reverse transcription loop-mediated isothermal amplification (RT–LAMP). By meeting diverse diagnostic and reagent needs, the platform yields testing results that closely align with those of commercial RT-LAMP and RTqPCR systems. Notable advantages of our system include its speed and cost-effectiveness. The assay is completed within 28min, including sample loading (5min), ribonucleic acid (RNA) extraction (3min), and RT-LAMP (20min). The cost of each assay is $9.5, and this pricing is competitive against that of Food and Drug Administration (FDA)-approved commercial alternatives. Although some RNA loss during on-chip extraction is observed, the platform maintains a potential limit of detection lower than 297 copies. Portability makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently located. Another key feature is the platform’s versatility, allowing users to choose between RTqPCR or RTLAMP tests based on specific requirements.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicrosystems & Nanoengineering. 2024, vol. 10, issue 1, p. 1-14.en
dc.identifier.doi10.1038/s41378-024-00677-6cs
dc.identifier.issn2055-7434cs
dc.identifier.orcid0000-0003-0564-1862cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-1232-2301cs
dc.identifier.other188684cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridA-6893-2013cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/249356
dc.language.isoencs
dc.publisherNaturecs
dc.relation.ispartofMicrosystems & Nanoengineeringcs
dc.relation.urihttps://www.nature.com/articles/s41378-024-00677-6cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2055-7434/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectmicrofluidicsen
dc.subjectnucleic acidsen
dc.subjectRT-PCRen
dc.subjectSARS-CoV-2en
dc.titleAn integrated microfluidic platform for nucleic acid testingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-188684en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:41:19en
sync.item.modts2025.01.17 16:39:10en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41378024006776.pdf
Size:
2.75 MB
Format:
Adobe Portable Document Format
Description:
file s41378024006776.pdf
Download
Collections
Ústav mikroelektroniky