An integrated microfluidic platform for nucleic acid testing
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Date
2024-05-23
Authors
Sun, Antao
Vopařilová, Petra
Liu, Xiaocheng
Kou, Bingqian
Řezníček, Tomáš
Lednický, Tomáš
Ni, Sheng
Kudr, Jiří
Zítka, Ondřej
Fohlerová, Zdenka
0000-0003-0564-1862Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
Nature
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Abstract
This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics. The system integrates the extraction and purification of nucleic acids, followed by amplification via either reverse transcription-quantitative polymerase chain reaction (RT–qPCR) or reverse transcription loop-mediated isothermal amplification (RT–LAMP). By meeting diverse diagnostic and reagent needs, the platform yields testing results that closely align with those of commercial RT-LAMP and RTqPCR systems. Notable advantages of our system include its speed and cost-effectiveness. The assay is completed within 28min, including sample loading (5min), ribonucleic acid (RNA) extraction (3min), and RT-LAMP (20min). The cost of each assay is $9.5, and this pricing is competitive against that of Food and Drug Administration (FDA)-approved commercial alternatives. Although some RNA loss during on-chip extraction is observed, the platform maintains a potential limit of detection lower than 297 copies. Portability makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently located. Another key feature is the platform’s versatility, allowing users to choose between RTqPCR or RTLAMP tests based on specific requirements.
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Citation
Microsystems & Nanoengineering. 2024, vol. 10, issue 1, p. 1-14.
https://www.nature.com/articles/s41378-024-00677-6
https://www.nature.com/articles/s41378-024-00677-6
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en