X-ray microtomography–based atlas of mouse cranial development

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dc.contributor.authorMatula, Jancs
dc.contributor.authorTesařová, Markétacs
dc.contributor.authorZikmund, Tomášcs
dc.contributor.authorKaucká, Markétacs
dc.contributor.authorAdameyko, Igorcs
dc.contributor.authorKaiser, Jozefcs
dc.coverage.issue3cs
dc.coverage.volume10cs
dc.date.accessioned2021-08-02T14:55:05Z
dc.date.available2021-08-02T14:55:05Z
dc.date.issued2021-03-02cs
dc.description.abstractX-ray microtomography (CT) has become an invaluable tool for non-destructive analysis of biological samples in the field of developmental biology. Mouse embryos are a typical model for investigation of human developmental diseases. By obtaining 3D high-resolution scans of the mouse embryo heads, we gain valuable morphological information about the structures prominent in the development of future face, brain, and sensory organs. The development of facial skeleton tracked in these CT data provides a valuable background for further studies of congenital craniofacial diseases and normal development. In this work, reusable tomographic data from 7 full 3D scans of mouse embryo heads are presented and made publicly available. The ages of these embryos range from E12.5 to E18.5. The samples were stained by phosphotungstic acid prior to scanning, which greatly enhanced the contrast of various tissues in the reconstructed images and enabled precise segmentation. The images were obtained on a laboratory-based CT system. Furthermore, we provide manually segmented masks of mesenchymal condensations (for E12.5 and E13.5) and cartilage present in the nasal capsule of the scanned embryos. We present a comprehensive dataset of X-ray 3D computed tomography images of the developing mouse head with high-quality manual segmentation masks of cartilaginous nasal capsules. The provided CT images can be used for studying any other major structure within the developing mouse heads. The high quality of the manually segmented models of nasal capsules may be instrumental to understanding the complex process of the development of the face in a mouse model.en
dc.formattextcs
dc.format.extent1-6cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationGigaScience. 2021, vol. 10, issue 3, p. 1-6.en
dc.identifier.doi10.1093/gigascience/giab012cs
dc.identifier.issn2047-217Xcs
dc.identifier.other171196cs
dc.identifier.urihttp://hdl.handle.net/11012/196504
dc.language.isoencs
dc.publisherOxford University Presscs
dc.relation.ispartofGigaSciencecs
dc.relation.urihttps://academic.oup.com/gigascience/article/10/3/giab012/6156288cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2047-217X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subject3D modellingen
dc.subjectcomputed tomographyen
dc.subjectmicrotomographyen
dc.subjectmouse embryo headen
dc.subjectnasal capsuleen
dc.subjecttissue contrasten
dc.subjectX-rayen
dc.titleX-ray microtomography–based atlas of mouse cranial developmenten
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-171196en
sync.item.dbtypeVAVen
sync.item.insts2021.08.02 16:55:05en
sync.item.modts2021.08.02 16:14:18en
thesis.grantorVysoké učení technické v Brně. Ústav soudního inženýrství. Ústav soudního inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé instrumentace a metody pro charakterizace materiálůcs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
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