Cell type specific adhesion to surfaces functionalised by amine plasma polymers

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dc.contributor.authorKřížková, Petracs
dc.contributor.authorJanů, Luciecs
dc.contributor.authorMedalová, Jiřinacs
dc.contributor.authorNečas, Davidcs
dc.contributor.authorMichlíček, Miroslavcs
dc.contributor.authorKaushik, Preetics
dc.contributor.authorPřibyl, Jancs
dc.contributor.authorBartošíková, Janacs
dc.contributor.authorManakhov, Antoncs
dc.contributor.authorBačáková, Luciecs
dc.contributor.authorZajíčková, Lenkacs
dc.coverage.issue1cs
dc.coverage.volume10cs
dc.date.issued2020-06-09cs
dc.description.abstractOur previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2020, vol. 10, issue 1, p. 1-14.en
dc.identifier.doi10.1038/s41598-020-65889-ycs
dc.identifier.issn2045-2322cs
dc.identifier.orcid0000-0002-6298-2228cs
dc.identifier.orcid0000-0001-7731-8453cs
dc.identifier.orcid0000-0002-6906-8906cs
dc.identifier.other165032cs
dc.identifier.researcheridAAD-5562-2022cs
dc.identifier.researcheridD-7166-2012cs
dc.identifier.researcheridE-3010-2012cs
dc.identifier.scopus22933742100cs
dc.identifier.urihttp://hdl.handle.net/11012/195753
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttps://www.nature.com/articles/s41598-020-65889-ycs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjecthuman-endothelial-cellsen
dc.subjecttissue transglutaminaseen
dc.subjectcollagenen
dc.subjectbiocompatibilityen
dc.subjectpolymerizationen
dc.subjectimmobilizationen
dc.subjectbiomaterialsen
dc.subjectglycocalyxen
dc.subjectresistanceen
dc.subjectnanofibersen
dc.titleCell type specific adhesion to surfaces functionalised by amine plasma polymersen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-165032en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:42:47en
sync.item.modts2025.01.17 16:51:37en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav teoretické a experimentální elektrotechnikycs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Vývoj metod analýzy a měřenícs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé nízkodimenzionální nanomateriálycs
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Pokročilé nízkodimenzionální nanomateriály
Vývoj metod analýzy a měření
Ústav teoretické a experimentální elektrotechniky