In Vivo Assessment on Freeze-Cast Calcium Phosphate-Based Scaffolds with a Selective Cell/Tissue Ingrowth
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Date
2024-10-21
Authors
Mařáková, Lucie
Pejchal, Jaroslav
Roleček, Jakub
Vojníková, Michaela
Chlup, Zdeněk
Mařák, Vojtěch
González-Sánchez, Manuela
Čížková, Jana
Salamon, David
0000-0002-2147-9917Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
AMER CHEMICAL SOC
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Abstract
Highly porous bioceramic scaffolds are widely used as bone substitutes in many applications. However, the use of bioceramics is often limited to hard tissues due to the risk of potential soft tissue calcification. A further limitation of highly porous bioceramic scaffolds is their poor mechanical stability, manifested by their tendency to break under stress. In our study, highly porous CaP-based scaffolds were prepared via freeze-casting with longitudinal and oriented pores ranging from 10 to 20 mu m and a relative porosity of similar to 70%. The resulting scaffolds achieved a flexural strength of 10.6 +/- 2.7 MPa, which, in conjunction with their favorable bioactivity, made them suitable for in vivo testing. The prepared scaffolds were subcutaneously implanted in rats for two distinct periods: 6 weeks and 6 months, respectively. The subsequent development of fibrous tissue and involvement of myofibroblasts, newly formed vessels, and macrophages were observed, with notable changes in spatial and temporal distributions within the implantation. The absence of calcification in the surrounding soft tissue, as a result of the narrow pore geometry, indicates the opportunity to tailor the scaffold behavior for soft tissue regeneration.
Description
Citation
ACS APPL MATER INTER. 2024, vol. 16, issue 43, p. 58326-58336.
https://pubs.acs.org/doi/10.1021/acsami.4c12715
https://pubs.acs.org/doi/10.1021/acsami.4c12715
Document type
Peer-reviewed
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Published version
Date of access to the full text
Language of document
en