Doubling Throughput of a Real-Time PCR
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Date
2015-07-10
Authors
Ahrberg, Christian D.
Neužil, Pavel
ORCID
Advisor
Referee
Mark
Journal Title
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Volume Title
Publisher
Macmillan Publishers
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Abstract
We added an intercallating dye to probe-based real-time PCR. One set of oligonucleotides had FAM probe while the second one was without. We were able to extract two DNA amplification curves using a single fluorescence channel
We added an intercallating dye to probe-based real-time PCR. One set of oligonucleotides had FAM probe while the second one was without. We were able to extract two DNA amplification curves using a single fluorescence channel
We added an intercallating dye to probe-based real-time PCR. One set of oligonucleotides had FAM probe while the second one was without. We were able to extract two DNA amplification curves using a single fluorescence channel
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Document type
Peer-reviewed
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Published version
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en