Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique
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Date
2015-10-28
Authors
Guszpit, Ewelina
Křížková, Soňa
Kepinska, Marta
Merlos Rodrigo, Miguel Ángel
Milnerowitz, Halina
Kopel, Pavel
Kizek, René
0000-0002-0479-8369Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
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Abstract
Quantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can be used as tumor biomarkers. These particles are a promising tool for selective, fast, andsensitive tagging and imaging in medicine. In thisstudy, an attempt was made to use QDs as a marker forhuman metallothionein (MT) isoforms 1 and 2. Fourkinds of CdTe QDs of different sizes bioconjugatedwith MT were analyzed using the chip-CE technique.Based on the results, it can be concluded that MT iswilling to interact with QDs, and the chip-CE technique enables the observation of their complexes.It was also observed that changes ranging roughly6–7 kDa, a value corresponding to the MT monomer,depend on the hydrodynamic diameters of QDs; also,the MT sample without cadmium interacted strongerwith QDs than MT saturated with cadmium. Resultsshow that MT is willing to interact with smaller QDs(blue CdTe) rather than larger ones QDs (red CdTe).To our knowledge, chip-CE has not previously beenapplied in the study of CdTe QDs interaction with MT.
Quantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can be used as tumor biomarkers. These particles are a promising tool for selective, fast, andsensitive tagging and imaging in medicine. In thisstudy, an attempt was made to use QDs as a marker forhuman metallothionein (MT) isoforms 1 and 2. Fourkinds of CdTe QDs of different sizes bioconjugatedwith MT were analyzed using the chip-CE technique.Based on the results, it can be concluded that MT iswilling to interact with QDs, and the chip-CE technique enables the observation of their complexes.It was also observed that changes ranging roughly6–7 kDa, a value corresponding to the MT monomer,depend on the hydrodynamic diameters of QDs; also,the MT sample without cadmium interacted strongerwith QDs than MT saturated with cadmium. Resultsshow that MT is willing to interact with smaller QDs(blue CdTe) rather than larger ones QDs (red CdTe).To our knowledge, chip-CE has not previously beenapplied in the study of CdTe QDs interaction with MT.
Quantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can be used as tumor biomarkers. These particles are a promising tool for selective, fast, andsensitive tagging and imaging in medicine. In thisstudy, an attempt was made to use QDs as a marker forhuman metallothionein (MT) isoforms 1 and 2. Fourkinds of CdTe QDs of different sizes bioconjugatedwith MT were analyzed using the chip-CE technique.Based on the results, it can be concluded that MT iswilling to interact with QDs, and the chip-CE technique enables the observation of their complexes.It was also observed that changes ranging roughly6–7 kDa, a value corresponding to the MT monomer,depend on the hydrodynamic diameters of QDs; also,the MT sample without cadmium interacted strongerwith QDs than MT saturated with cadmium. Resultsshow that MT is willing to interact with smaller QDs(blue CdTe) rather than larger ones QDs (red CdTe).To our knowledge, chip-CE has not previously beenapplied in the study of CdTe QDs interaction with MT.
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Citation
JOURNAL OF NANOPARTICLE RESEARCH. 2015, vol. 17, issue 11, p. 423-1-423-12.
https://link.springer.com/article/10.1007%2Fs11051-015-3226-8
https://link.springer.com/article/10.1007%2Fs11051-015-3226-8
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Peer-reviewed
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en