Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique

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dc.contributor.authorGuszpit, Ewelinacs
dc.contributor.authorKřížková, Soňacs
dc.contributor.authorKepinska, Martacs
dc.contributor.authorMerlos Rodrigo, Miguel Ángelcs
dc.contributor.authorMilnerowitz, Halinacs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue11cs
dc.coverage.volume17cs
dc.date.issued2015-10-28cs
dc.description.abstractQuantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can be used as tumor biomarkers. These particles are a promising tool for selective, fast, andsensitive tagging and imaging in medicine. In thisstudy, an attempt was made to use QDs as a marker forhuman metallothionein (MT) isoforms 1 and 2. Fourkinds of CdTe QDs of different sizes bioconjugatedwith MT were analyzed using the chip-CE technique.Based on the results, it can be concluded that MT iswilling to interact with QDs, and the chip-CE technique enables the observation of their complexes.It was also observed that changes ranging roughly6–7 kDa, a value corresponding to the MT monomer,depend on the hydrodynamic diameters of QDs; also,the MT sample without cadmium interacted strongerwith QDs than MT saturated with cadmium. Resultsshow that MT is willing to interact with smaller QDs(blue CdTe) rather than larger ones QDs (red CdTe).To our knowledge, chip-CE has not previously beenapplied in the study of CdTe QDs interaction with MT.en
dc.description.abstractQuantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can be used as tumor biomarkers. These particles are a promising tool for selective, fast, andsensitive tagging and imaging in medicine. In thisstudy, an attempt was made to use QDs as a marker forhuman metallothionein (MT) isoforms 1 and 2. Fourkinds of CdTe QDs of different sizes bioconjugatedwith MT were analyzed using the chip-CE technique.Based on the results, it can be concluded that MT iswilling to interact with QDs, and the chip-CE technique enables the observation of their complexes.It was also observed that changes ranging roughly6–7 kDa, a value corresponding to the MT monomer,depend on the hydrodynamic diameters of QDs; also,the MT sample without cadmium interacted strongerwith QDs than MT saturated with cadmium. Resultsshow that MT is willing to interact with smaller QDs(blue CdTe) rather than larger ones QDs (red CdTe).To our knowledge, chip-CE has not previously beenapplied in the study of CdTe QDs interaction with MT.en
dc.formattextcs
dc.format.extent423-1-423-12cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJOURNAL OF NANOPARTICLE RESEARCH. 2015, vol. 17, issue 11, p. 423-1-423-12.en
dc.identifier.doi10.1007/s11051-015-3226-8cs
dc.identifier.issn1388-0764cs
dc.identifier.orcid0000-0002-0479-8369cs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.other121230cs
dc.identifier.researcheridE-9617-2012cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.scopus6603604023cs
dc.identifier.urihttp://hdl.handle.net/11012/194770
dc.language.isoencs
dc.publisherSpringercs
dc.relation.ispartofJOURNAL OF NANOPARTICLE RESEARCHcs
dc.relation.urihttps://link.springer.com/article/10.1007%2Fs11051-015-3226-8cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1388-0764/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectBioconjugationen
dc.subjectChip-CEen
dc.subjectMetallothioneinen
dc.subjectQuantum dotsen
dc.subjectBiomarkersen
dc.subjectHealth effectsen
dc.subjectBioconjugation
dc.subjectChip-CE
dc.subjectMetallothionein
dc.subjectQuantum dots
dc.subjectBiomarkers
dc.subjectHealth effects
dc.titleFluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE techniqueen
dc.title.alternativeFluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE techniqueen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-121230en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:16:37en
sync.item.modts2025.10.14 10:45:12en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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