Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis

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dc.contributor.authorVaněčková, Terezacs
dc.contributor.authorZítka, Jancs
dc.contributor.authorHlaváček, Antoníncs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorVaculovičová, Markétacs
dc.date.issued2017-12-31cs
dc.description.abstractUpconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.en
dc.description.abstractUpconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.en
dc.formattextcs
dc.format.extent953-957cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2017. 2017, p. 953-957.en
dc.identifier.isbn978-80-7509-529-9cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0002-6771-1304cs
dc.identifier.other148779cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridE-5583-2016cs
dc.identifier.urihttp://hdl.handle.net/11012/84178
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2017cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201701-0113_Characterization-of-upconversion-nanoparticles-by-fluorescence-spectrometry-and-capillary-electrophoresis.php?back=/magno/mnt/2017/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectUpconversion nanoparticlesen
dc.subjectUCNPen
dc.subjectfluorescence spectrometryen
dc.subjectcapillary electrophoresisen
dc.subjectUpconversion nanoparticles
dc.subjectUCNP
dc.subjectfluorescence spectrometry
dc.subjectcapillary electrophoresis
dc.titleCharacterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresisen
dc.title.alternativeCharacterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresisen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-148779en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:17:16en
sync.item.modts2025.10.14 09:40:18en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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