Biopolymeric fibers prepared by centrifugal spinning blended with ZnO nanoparticles for the treatment of Acne vulgaris

Abstract

Acne vulgaris is a serious dermatological disease affecting a significant part of the population. Currently, available therapeutics are effective only at high concentrations, which has a negative environmental and economic impact. In particular, ZnO nanoparticles (NPs) have a great potential in various biomedical applications due to their specific properties and antibacterial/antiviral activity. In this study, biomedically approved ZnO NPs with distinct diameter were used as the active therapeutic modality to treat acne-causing pathogens. For the first time, we show the utilization of ZnO NPs that were evenly distributed within centrifugally spun fiber carriers. Upon application on the skin, ZnO NPs can sustainably release and have profound antibacterial activity at lower therapeutic concentrations. Fibers were made using innovative centrifugal spinning procedure from natural polymers - gum arabic and pullulan - that are known for their biocompatibility. Different amount of ZnO NPs (from 0.03 to 4.5 wt.% related to the dry mass) was added into the spinning polymer solution, either in a form of a dry powder or as a dispersion containing NPs and isopropyl myristate. The resulting fibers were subsequently characterized for morphology and presence of ZnO NPs by Scanning Electron Microscopy and Energy-Dispersive X-ray fluorescence spectrometry. The materials were thoroughly assessed for their antibacterial activity against Cutibacterium acnes and Staphylococcus epidermidis , which are major opportunistic pathogens causing acne. The combination of two types of nanomaterials, namely active nanoparticles and fiber carriers, proved to be very promising and bear a great potential for the treatment of these diseases.Acne vulgaris is a serious dermatological disease affecting a significant part of the population. Currently, available therapeutics are effective only at high concentrations, which has a negative environmental and economic impact. In particular, ZnO nanoparticles (NPs) have a great potential in various biomedical applications due to their specific properties and antibacterial/antiviral activity. In this study, biomedically approved ZnO NPs with distinct diameter were used as the active therapeutic modality to treat acne-causing pathogens. For the first time, we show the utilization of ZnO NPs that were evenly distributed within centrifugally spun fiber carriers. Upon application on the skin, ZnO NPs can sustainably release and have profound antibacterial activity at lower therapeutic concentrations. Fibers were made using innovative centrifugal spinning procedure from natural polymers - gum arabic and pullulan - that are known for their biocompatibility. Different amount of ZnO NPs (from 0.03 to 4.5 wt.% related to the dry mass) was added into the spinning polymer solution, either in a form of a dry powder or as a dispersion containing NPs and isopropyl myristate. The resulting fibers were subsequently characterized for morphology and presence of ZnO NPs by Scanning Electron Microscopy and Energy-Dispersive X-ray fluorescence spectrometry. The materials were thoroughly assessed for their antibacterial activity against Cutibacterium acnes and Staphylococcus epidermidis , which are major opportunistic pathogens causing acne. The combination of two types of nanomaterials, namely active nanoparticles and fiber carriers, proved to be very promising and bear a great potential for the treatment of these diseases.