Highly selective and sensitive electrochemical biosensor based on electrochemically reduced graphene oxide for detection of miRNA as a cancer biomarker

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dc.contributor.authorVaňová, Veronikacs
dc.contributor.authorSedláčková, Eliškacs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorBytešníková, Zuzanacs
dc.contributor.authorAdam, Vojtěchcs
dc.date.accessioned2021-05-04T10:54:20Z
dc.date.available2021-05-04T10:54:20Z
dc.date.issued2020-06-10cs
dc.description.abstractNowadays, cancer is one of the most outrageous diseases affecting millions of people throughout the world. In this study, the sensitive biosensor for miRNA-21 detection was developed. Through a facile sulfonamide coupling reaction a miRNA-21 specific DNA hybridization probe was immobilized onto a glassy carbon electrode (GCE) modified with electrochemical reduced graphene oxide (erGO). miRNA is a small non-coding RNA molecule which plays an important role in gene expression. The level of miRNA in extracellular fluids varying in the presence of cancer and it can serve as potential biomarker for early detection of cancer. An elevated level of miRNA-21 represents a common feature of pathological cell growth or cell stress and is connected with almost all types of cancer and with cardiovascular diseases, as well. The aim of this study was improve the electrochemical properties by using erGO as a modification for better sensitivity of the developed biosensor. After the modification with erGO, 1-amino-2-naphthol-4-sulfonic acid (AN-SO3-) was electrodeposited on the GCE surface. --Finally, the amino terminated capture probe was immobilized on the GCE via the sulfonamide coupling reaction. The developed biosensor for the detection of specific miRNA sequence was optimized and modified for using in further experiments.en
dc.formattextcs
dc.format.extent385-390cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNANOCON 2019 CONFERENCE PROCEEDINGS. 2020, p. 385-390.en
dc.identifier.doi10.37904/nanocon.2019.8615cs
dc.identifier.isbn978-80-87294-95-6cs
dc.identifier.other164271cs
dc.identifier.urihttp://hdl.handle.net/11012/196676
dc.language.isoencs
dc.publisherTangercs
dc.relation.ispartofNANOCON 2019 CONFERENCE PROCEEDINGScs
dc.relation.urihttps://doi.org/10.37904/nanocon.2019.8615cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectReduced graphene oxideen
dc.subjectmiRNAen
dc.subjectbiosensoren
dc.subjectelectrochemical detectionen
dc.titleHighly selective and sensitive electrochemical biosensor based on electrochemically reduced graphene oxide for detection of miRNA as a cancer biomarkeren
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-164271en
sync.item.dbtypeVAVen
sync.item.insts2022.03.03 16:55:15en
sync.item.modts2022.03.03 16:15:15en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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