Comparison of metal nanoparticles (Au, Ag, Eu, Cd) used for immunoanalysis using LA-ICP-MS detection

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dc.contributor.authorVlčnovská, Marcelacs
dc.contributor.authorŠtossová, Anetacs
dc.contributor.authorKuchynka, Michaelacs
dc.contributor.authorDillingerová, Veronikacs
dc.contributor.authorPolanská, Hanacs
dc.contributor.authorMasařík, Michalcs
dc.contributor.authorHrstka, Romancs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKanický, Viktorcs
dc.contributor.authorVaculovič, Tomášcs
dc.contributor.authorVaculovičová, Markétacs
dc.coverage.issue3cs
dc.coverage.volume26cs
dc.date.accessioned2021-05-06T14:55:32Z
dc.date.available2021-05-06T14:55:32Z
dc.date.issued2021-02-01cs
dc.description.abstractImmunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1-14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.en
dc.formattextcs
dc.format.extent246-259cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMOLECULES. 2021, vol. 26, issue 3, p. 246-259.en
dc.identifier.doi10.3390/molecules26030630cs
dc.identifier.issn1420-3049cs
dc.identifier.other171012cs
dc.identifier.urihttp://hdl.handle.net/11012/196717
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMOLECULEScs
dc.relation.urihttps://www.mdpi.com/1420-3049/26/3/630cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1420-3049/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectprotein p53en
dc.subjectdot-bloten
dc.subjectantibodyen
dc.titleComparison of metal nanoparticles (Au, Ag, Eu, Cd) used for immunoanalysis using LA-ICP-MS detectionen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-171012en
sync.item.dbtypeVAVen
sync.item.insts2021.05.06 16:55:32en
sync.item.modts2021.05.06 16:14:47en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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