Vliv složení lipidů na přípravu podpořených lipidových dvojvrstev

Abstract

podpořené lipidové dvojvrstvy, model biomembrány, DOPC, DPPC, lecithin, cholesterol, fluorescenční spektroskopie, fluorescenční mikroskopie

This bachelor's thesis focuses on the preparation of supported lipid bilayers (SLBs) using the vesicle fusion method and investigates the effect of lipid composition on their formation. Phospholipids DOPC, DPPC, and cholesterol were used in various ratios, along with a commercially available mixture of egg lecithin phospholipids representing a model of a real biological membrane. The lipid composition had a significant influence on the physicochemical properties of the vesicles, and consequently on the quality of the resulting SLBs. Fluorescence spectroscopy with Laurdan and DPH probes was used to study fluorescence anisotropy and generalized polarization. Dynamic and electrophoretic light scattering were employed as complementary methods to characterize vesicle size and zeta potential. The formed SLBs were visualized using fluorescence microscopy with the DiI probe. It was found that higher contents of unsaturated phospholipids and cholesterol led to more defects in the bilayer. In contrast, mixtures with a higher proportion of saturated phospholipids and without cholesterol exhibited more homogeneous regions.