A simple technique for assessing the cuticular diffusion of humic acid biostimulants

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dc.contributor.authorSmilková, Marcelacs
dc.contributor.authorSmilek, Jiřícs
dc.contributor.authorKalina, Michalcs
dc.contributor.authorKlučáková, Martinacs
dc.contributor.authorPekař, Miloslavcs
dc.contributor.authorSedláček, Petrcs
dc.coverage.issue1cs
dc.coverage.volume15cs
dc.date.issued2019-07-31cs
dc.description.abstractBackground: Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing of physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration on humates through plant cuticles is proposed, tested, and evaluated. Results: The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amounts of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested. Conclusions: The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.en
dc.formattextcs
dc.format.extent1-11cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLANT METHODS. 2019, vol. 15, issue 1, p. 1-11.en
dc.identifier.doi10.1186/s13007-019-0469-xcs
dc.identifier.issn1746-4811cs
dc.identifier.orcid0000-0002-6553-1764cs
dc.identifier.orcid0000-0002-4224-0841cs
dc.identifier.orcid0000-0003-4969-6988cs
dc.identifier.orcid0000-0002-3878-0917cs
dc.identifier.orcid0000-0002-6211-9643cs
dc.identifier.other157782cs
dc.identifier.scopus55844628200cs
dc.identifier.scopus7006307069cs
dc.identifier.scopus25621752900cs
dc.identifier.urihttp://hdl.handle.net/11012/180811
dc.language.isoencs
dc.publisherBMCcs
dc.relation.ispartofPLANT METHODScs
dc.relation.urihttps://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1746-4811/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectDiffusionen
dc.subjectHydrogelen
dc.subjectPrunus laurocerasusen
dc.subjectLiquid fertilizationen
dc.subjectHumic substancesen
dc.subjectPlant cuticleen
dc.titleA simple technique for assessing the cuticular diffusion of humic acid biostimulantsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-157782en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:37:53en
sync.item.modts2025.01.17 15:21:24en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemiecs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
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