Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

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dc.contributor.authorBuchtová, Žanetacs
dc.contributor.authorLacková, Zuzanacs
dc.contributor.authorKudr, Jiřícs
dc.contributor.authorZítka, Zdeněkcs
dc.contributor.authorŠkoda, Jancs
dc.contributor.authorZítka, Ondřejcs
dc.coverage.issue10cs
dc.coverage.volume23cs
dc.date.accessioned2020-08-04T11:03:43Z
dc.date.available2020-08-04T11:03:43Z
dc.date.issued2018-10-01cs
dc.description.abstractGlutathione (gamma-glutamyl-cysteinyl-glycine; also known as GSH) is an endogenous antioxidant that plays a crucial role in cell defense mechanisms against oxidative stress. It is thus not surprising that this molecule can serve as a biomarker for oxidative stress monitoring. As capillary blood is a highly accessible target for biomarking, it is a valuable bodily fluid for diagnosing human GSH levels. This study focused on the optimization of GSH measurements from micro volumes of capillary blood prior to using electrochemical detection. The optimization of experimental parameters, including the sample volume and its stability, was performed and evaluated. Moreover, we tested the optimized method as part of a short-term study. The study consisted of examining 10 subjects within 96 h of their consumption of high amounts of antioxidants, attained from a daily dose of 2 g/150 mL of green tea. The subjects' capillary blood (5 mu L) was taken at 0 h, 48 h, and 96 h for subsequent analysis. The short-term supplementation of diet with green tea showed an increase of GSH pool by approximately 38% (between 0 and 48 h) within all subjects.en
dc.formattextcs
dc.format.extent2504-1-2504-10cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMOLECULES. 2018, vol. 23, issue 10, p. 2504-1-2504-10.en
dc.identifier.doi10.3390/molecules23102504cs
dc.identifier.issn1420-3049cs
dc.identifier.other152159cs
dc.identifier.urihttp://hdl.handle.net/11012/180500
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMOLECULEScs
dc.relation.urihttp://www.mdpi.com/1420-3049/23/10/2504cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1420-3049/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectantioxidant moleculesen
dc.subjectblood drop analysisen
dc.subjectelectrochemical analysisen
dc.subjectnutritional studyen
dc.subjectsample pretreatmenten
dc.titleCapillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-152159en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:43en
sync.item.modts2020.08.04 12:16:22en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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