Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

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dc.contributor.authorBalvan, Jancs
dc.contributor.authorKřížová, Anetacs
dc.contributor.authorGumulec, Jaromírcs
dc.contributor.authorRaudenská, Martinacs
dc.contributor.authorSládek, Zbyšekcs
dc.contributor.authorSedláčková, Miroslavacs
dc.contributor.authorBabula, Petrcs
dc.contributor.authorSvobodová, Markétacs
dc.contributor.authorKizek, Renécs
dc.contributor.authorChmelík, Radimcs
dc.contributor.authorMasařík, Michalcs
dc.coverage.issue3cs
dc.coverage.volume10cs
dc.date.issued2015-03-24cs
dc.description.abstractIdentification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection Multimodal holographic microscopy (MHM). The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.en
dc.description.abstractIdentification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection Multimodal holographic microscopy (MHM). The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.en
dc.formattextcs
dc.format.extent1-16cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLoS One. 2015, vol. 10, issue 3, p. 1-16.en
dc.identifier.doi10.1371/journal.pone.0121674cs
dc.identifier.issn1932-6203cs
dc.identifier.orcid0000-0002-9919-6204cs
dc.identifier.orcid0000-0002-9658-3444cs
dc.identifier.orcid0000-0001-8529-552Xcs
dc.identifier.orcid0000-0001-5410-4794cs
dc.identifier.orcid0000-0003-1172-7195cs
dc.identifier.other113695cs
dc.identifier.researcheridG-8475-2014cs
dc.identifier.researcheridD-7638-2012cs
dc.identifier.researcheridD-7616-2012cs
dc.identifier.researcheridD-9920-2012cs
dc.identifier.scopus55769747816cs
dc.identifier.scopus6603192372cs
dc.identifier.urihttp://hdl.handle.net/11012/193259
dc.language.isoencs
dc.publisherPLOScs
dc.relation.ispartofPLoS Onecs
dc.relation.urihttps://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectMultimodal holographic microscopyen
dc.subjectapoptosisen
dc.subjectoncosisen
dc.subjectnecrosisen
dc.subjectcell deathen
dc.subjectplumbaginen
dc.subjectflow cytometryen
dc.subjecttransmission electron microscopyen
dc.subjectMultimodal holographic microscopy
dc.subjectapoptosis
dc.subjectoncosis
dc.subjectnecrosis
dc.subjectcell death
dc.subjectplumbagin
dc.subjectflow cytometry
dc.subjecttransmission electron microscopy
dc.titleMultimodal Holographic Microscopy: Distinction between Apoptosis and Oncosisen
dc.title.alternativeMultimodal Holographic Microscopy: Distinction between Apoptosis and Oncosisen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-113695en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:16:36en
sync.item.modts2025.10.14 10:42:19en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Experimentální biofotonikacs
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