A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode

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dc.contributor.authorVlachová, Janacs
dc.contributor.authorTmejová, Kateřinacs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorKorabík, Michalcs
dc.contributor.authorZítka, Jancs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorKynický, Jindřichcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue2cs
dc.coverage.volume15cs
dc.date.accessioned2020-08-04T11:04:04Z
dc.date.available2020-08-04T11:04:04Z
dc.date.issued2015-02-01cs
dc.description.abstractModification of carbon materials, especially graphene-based materials, has wide applications in electrochemical detection such as electrochemical lab-on-chip devices. A glassy carbon electrode (GCE) modified with chemically alternated graphene oxide was used as a working electrode (glassy carbon modified by graphene oxide with sulphur containing compounds and Nafion) for detection of nucleobases in hydrolysed samples (HCl pH = 2.9, 100 °C, 1 h, neutralization by NaOH). It was found out that modification, especially with trithiocyanuric acid, increased the sensitivity of detection in comparison with pure GCE. All processes were finally implemented in a microfluidic chip formed with a 3D printer by fused deposition modelling technology. As a material for chip fabrication, acrylonitrile butadiene styrene was chosen because of its mechanical and chemical stability. The chip contained the one chamber for the hydrolysis of the nucleic acid and another for the electrochemical detection by the modified GCE. This chamber was fabricated to allow for replacement of the GCE.en
dc.formattextcs
dc.format.extent2438-2452cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2015, vol. 15, issue 2, p. 2438-2452.en
dc.identifier.doi10.3390/s150202438cs
dc.identifier.issn1424-8220cs
dc.identifier.other123331cs
dc.identifier.urihttp://hdl.handle.net/11012/63725
dc.language.isoencs
dc.publisherMDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLANDcs
dc.relation.ispartofSENSORScs
dc.relation.urihttp://www.mdpi.com/1424-8220/15/2/2438cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectREDUCED GRAPHENE OXIDE; TRITHIOCYANURIC ACID; 2en
dc.subject4en
dc.subject6-TRIMERCAPTO-1en
dc.subject3en
dc.subject5-TRIAZINE TMT; FUNCTIONALIZED GRAPHENE; COMPLEXES; CHEMISTRY; SALT; 1en
dc.subject3en
dc.subject5-TRIAZINE-2en
dc.subject4en
dc.subject6-TRITHIOLen
dc.subjectTRIMERCAPTOTRIAZINEen
dc.subjectINHIBITIONen
dc.titleA 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrodeen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-123331en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:04:04en
sync.item.modts2020.08.04 12:26:55en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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