Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients Swab

Simple item page
dc.contributor.authorKomínková, Markétacs
dc.contributor.authorMichálek, Petrcs
dc.contributor.authorČíhalová, Kristýnacs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorNejdl, Lukášcs
dc.contributor.authorŠmerková, Kristýnacs
dc.contributor.authorDostálová, Simonacs
dc.contributor.authorHegerová, Dagmarcs
dc.contributor.authorHeger, Zbyněkcs
dc.contributor.authorVeselý, Radekcs
dc.contributor.authorGumulec, Jaromírcs
dc.contributor.authorKynický, Jindřichcs
dc.contributor.authorXhaxhiu, Kledics
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue4cs
dc.coverage.volume16cs
dc.date.issued2015-03-31cs
dc.description.abstractIn this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (y-glutamylcysteine synthase-GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 uM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of “more resistant" E. coli exhibited differences in Km between 0.3 and 0.8 uM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.en
dc.description.abstractV této práci jsme se zaměřili na rozdíly mezi bakteriálními kulturami E. coli získanými z výtěrů infekčních ran pacientů ve srovnání s laboratorními E. coli. Kromě toho, blokování proteinu odpovědného za syntézu glutathionu (y-glutamylcysteingylcin) a glutathion systetázy (GCL) bylo zkoumáno za použití 10 mM buthionin sulfoximinu. Každá E. coli ukázala výrazné rozdíly v rezistenci vůči antibiotikům. Podle odolnosti proti E. coli, byly E. coli rozděleny do experimentálních skupin na základě statistického vyhodnocení jejich vlastnosté, zda jsou odolnější či citlivější. Tyto skupiny byly také použity pro nalezení rozdílů v závislosti glutathionové dráhy a rezistenci na antibiotika. Více citlivé E. coli vykazovaly stejné kinetiky syntézy glutathionu zatímco GCL blokujecs
dc.formattextcs
dc.format.extent7210-7229cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Molecular Sciences. 2015, vol. 16, issue 4, p. 7210-7229.en
dc.identifier.doi10.3390/ijms16047210cs
dc.identifier.issn1422-0067cs
dc.identifier.orcid0000-0002-0121-0128cs
dc.identifier.orcid0000-0002-2912-714Xcs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0001-6332-8917cs
dc.identifier.orcid0000-0002-1667-7660cs
dc.identifier.orcid0000-0003-0826-234Xcs
dc.identifier.orcid0000-0002-3915-7270cs
dc.identifier.orcid0000-0002-9658-3444cs
dc.identifier.orcid0000-0001-9193-8990cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other114271cs
dc.identifier.researcheridB-9180-2014cs
dc.identifier.researcheridC-2610-2016cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridJ-7030-2012cs
dc.identifier.researcheridD-1973-2013cs
dc.identifier.researcheridD-7638-2012cs
dc.identifier.researcheridC-1091-2014cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus55769747816cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/63716
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Molecular Sciencescs
dc.relation.urihttp://www.mdpi.com/1422-0067/16/4/7210cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1422-0067/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectbuthionine sulfoximineen
dc.subjectEscherichia colien
dc.subjectglutathioneen
dc.subjectinfectionsen
dc.subjectswabsen
dc.titleStudy of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients Swaben
dc.title.alternativeStudie vazby mezi Glutathionovou cestou a rezistencí vůči antibiotikům Escherichia coli od pacientů pomocí tampónového výtěrucs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-114271en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:18en
sync.item.modts2025.01.17 15:16:38en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijms1607210.pdf
Size:
2.73 MB
Format:
Adobe Portable Document Format
Description:
ijms1607210.pdf
Download
Collections
Chytré nanonástroje