Using QD-FRET based method to investigate protein-protein interactions

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dc.contributor.authorPavelicová, Kristýnacs
dc.contributor.authorZemánková, Kristýnacs
dc.contributor.authorBezděková, Jaroslavacs
dc.contributor.authorVaculovičová, Markétacs
dc.date.accessioned2021-07-14T14:55:52Z
dc.date.available2021-07-14T14:55:52Z
dc.date.issued2021-07-12cs
dc.description.abstractFörster resonance energy transfer (FRET) is an energy transfer process between a pair of light-sensitive molecules, where the donor fluorophore (initially in its electronic excited state) transfers energy to an acceptor chromophore. In this study, FRET was used to investigate the dimerization of metallothionein isoform MT-1A. The FRET system was developed based on a fluorescent quantum dots (QDs) and cyanine dye 3.5 (Cy3.5) as a versatile tool to probe small distance changes between acceptor and donor fluorophores in nanometer range. Herein, the water-soluble 450-nm emitting QDs as the donor and 590-nm Cy3.5 as the acceptor were covalently conjugated with MT-1 according to the protocol. Previous studies suggest that MTs may form oligomers under certain conditions. Therefore, further studies of this phenomenon, which has been studied here using capillary electrophoresis (CE) coupled with fluorescence detection.en
dc.formattextcs
dc.format.extent390-394cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationProceedings 12th International Conference on Nanomaterials - Research & Application. 2021, p. 390-394.en
dc.identifier.doi10.37904/nanocon.2020.3750cs
dc.identifier.isbn978-80-87294-98-7cs
dc.identifier.issn2694-930Xcs
dc.identifier.other172013cs
dc.identifier.urihttp://hdl.handle.net/11012/200498
dc.language.isoencs
dc.publisherTangercs
dc.relation.ispartofProceedings 12th International Conference on Nanomaterials - Research & Applicationcs
dc.relation.urihttps://www.confer.cz/nanocon/2020/3750-using-qd-fret-based-method-to-investigate-protein-protein-interactionscs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2694-930X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectFörster resonance energy transfer (FRET)en
dc.subjectquantum dots (QDs)en
dc.subjectOligomerizationen
dc.subjectCapillary electrophoresisen
dc.subjectMetallothioneinen
dc.titleUsing QD-FRET based method to investigate protein-protein interactionsen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-172013en
sync.item.dbtypeVAVen
sync.item.insts2021.11.14 16:59:47en
sync.item.modts2021.11.14 16:16:22en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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