Modulation of Induced Cytotoxicity of Doxorubicin by Using Apoferritin and Liposomal Cages

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dc.contributor.authorGumulec, Jaromírcs
dc.contributor.authorFojtů, Michaelacs
dc.contributor.authorRaudenská, Martinacs
dc.contributor.authorSztalmachová, Markétacs
dc.contributor.authorSkotáková, Annacs
dc.contributor.authorVlachová, Janacs
dc.contributor.authorSkaličková, Sylviecs
dc.contributor.authorNejdl, Lukášcs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorKnopfová, Luciacs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKizek, Renécs
dc.contributor.authorStiborová, Mariecs
dc.contributor.authorBabula, Petrcs
dc.contributor.authorMasařík, Michalcs
dc.coverage.issue12cs
dc.coverage.volume15cs
dc.date.accessioned2020-08-04T11:03:51Z
dc.date.available2020-08-04T11:03:51Z
dc.date.issued2014-12-01cs
dc.description.abstractDoxorubicin is an effective chemotherapeutic drug, however, its toxicity is a significant limitation in therapy. Encapsulation of doxorubicin inside liposomes or ferritin cages decreases cardiotoxicity while maintaining anticancer potency. We synthesized novel apoferritin-and liposome-encapsulated forms of doxorubicin ("Apodox" and "lip-8-dox") and compared its toxicity with doxorubicin and Myocet on prostate cell lines. Three different prostatic cell lines PNT1A, 22Rv1, and LNCaP were chosen. The toxicity of the modified doxorubicin forms was compared to conventional doxorubicin using the MTT assay, real-time cell impedance-based cell growth method (RTCA), and flow cytometry. The efficiency of doxorubicin entrapment was 56% in apoferritin cages and 42% in the liposome carrier. The accuracy of the RTCA system was verified by flow-cytometric analysis of cell viability. The doxorubicin half maximal inhibition concentrations (IC50) were determined as 170.5, 234.0, and 169.0 nM for PNT1A, 22Rv1, and LNCaP, respectively by RTCA. Lip8-dox is less toxic on the non-tumor cell line PNT1A compared to doxorubicin, while still maintaining the toxicity to tumorous cell lines similar to doxorubicin or epirubicin (IC50 = 2076.7 nM for PNT1A vs. 935.3 and 729.0 nM for 22Rv1 and LNCaP). Apodox IC50 was determined as follows: 603.1, 1344.2, and 931.2 nM for PNT1A, 22Rv1, and LNCaP.en
dc.formattextcs
dc.format.extent22960-22977cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Molecular Sciences. 2014, vol. 15, issue 12, p. 22960-22977.en
dc.identifier.doi10.3390/ijms151222960cs
dc.identifier.issn1422-0067cs
dc.identifier.other145129cs
dc.identifier.urihttp://hdl.handle.net/11012/189391
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Molecular Sciencescs
dc.relation.urihttps://www.mdpi.com/1422-0067/15/12/22960cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1422-0067/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectdoxorubicinen
dc.subjectliposomeen
dc.subjectapoferritinen
dc.subjectcanceren
dc.subjectcardiotoxicityen
dc.subjectmodificationen
dc.subjectencapsulationen
dc.titleModulation of Induced Cytotoxicity of Doxorubicin by Using Apoferritin and Liposomal Cagesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-145129en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:51en
sync.item.modts2020.08.04 12:31:49en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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