Distance-based paper microfluidic devices for rapid visual quantification of heavy metals in herbal supplements and cosmetics

Manmana, Yanawut; Macka, Miroslav; Nuchtavorn, Nantana

Distance-based paper microfluidic devices for rapid visual quantification of heavy metals in herbal supplements and cosmetics

Files

d4ra05358c.pdf(881.36 KB)

Date

2024-11-11

Authors

Manmana, Yanawut

Macka, Miroslav

Nuchtavorn, Nantana

ORCID

0000-0002-6792-2574

Publisher

Royal Society of Chemistry

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Abstract

Distance-based detection (DbD) on paper-based microfluidic analytical devices (mu PADs) has emerged as a promising, cost-effective, simple, and instrumentation-free assay method. Broadening the applicability of a new way of immobilization of reagent for DbD on mu PADs (D mu PADs) is presented, employing an ion exchange (IE) interaction of an anionic metallochromic reagent, 2-(5-bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol (5-Br-PAPS), on the anion-exchange filter paper. The IE D mu PADs demonstrate superiority over standard cellulose filter paper in terms of the degree of reagent immobilization, detection sensitivity, and clear detection endpoints due to the strong retention of 5-Br-PAPS. The study investigated various parameters influencing DbD, including 5-Br-PAPS concentrations (0.25-1 mM), buffer types (acetic acid-Tris, MES), buffer concentrations (20-500 mM), and auxiliary complexing agents (acetic, formic, and glycolic acids). Subsequently, the performance of 17 metals (Ag+, Cd2+, Co2+, Cr3+, Cu2+, Fe2+, Hg2+, La2+, Mn2+, Ni2+, Pb2+, Ti2+, Zn2+, Al3+, As3+, Fe3+, and V4+) was evaluated, with color formation observed for 12 metals. Additionally, the paper surface was examined using SEM and SEM-EDX to verify the suitability of certain areas in the detection channel for reagent immobilization and metal binding. This method demonstrates quantitation limits of metals in the low mu g mL-1 range, showing great potential for the rapid screening of toxic metals commonly found in herbal supplements and cosmetics regulated by the Food and Drug Administration (FDA). Thus, it holds promise for enhancing safety and regulatory compliance in product quality assessment. Furthermore, this method offers a cost-effective, environmentally sustainable, and user-friendly approach for the rapid visual quantification of heavy metals for in-field analysis, eliminating the need for complex instrumentation.

Keywords

SEPARATION, SURFACE, IONS

Citation

RSC Advances. 2024, vol. 14, issue 49, p. 36142-36151.
https://pubs.rsc.org/en/content/articlelanding/2024/ra/d4ra05358c

Document type

Peer-reviewed

Document version

Published version

Language of document

en

Document licence

Creative Commons Attribution-NonCommercial 3.0 Unported
http://creativecommons.org/licenses/by-nc/3.0/